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1
A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.当以反式提供开放阅读框2（ORF2）时，果蝇中的一个基因标记I元件可以被激活。
Genetics. 1998 Jan;148(1):267-75. doi: 10.1093/genetics/148.1.267.
2
A genetically tagged, defective I element can be complemented by actively transposing I factors in the germline of I-R dysgenic females in Drosophila melanogaster.一个带有基因标记的缺陷型I因子，可以被黑腹果蝇中I-R杂种不育雌性种系中活跃转座的I因子所互补。
Mol Gen Genet. 1995 Aug 30;248(4):434-8. doi: 10.1007/BF02191643.
3
New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.关于果蝇中含有ORF1的转基因对I因子活性的同源依赖性沉默的新见解。
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4
Trans-complementation of an endonuclease-defective tagged I element as a tool for the study of retrotransposition in Drosophila melanogaster.作为研究黑腹果蝇反转录转座工具的内切核酸酶缺陷型标记I元件的反式互补作用
Mol Genet Genomics. 2002 Aug;267(6):829-34. doi: 10.1007/s00438-002-0716-z. Epub 2002 Jul 9.
5
High-frequency retrotransposition of a marked I factor in Drosophila melanogaster correlates with a dynamic expression pattern of the ORF1 protein in the cytoplasm of oocytes.果蝇中一个标记的I因子的高频逆转座与卵母细胞细胞质中ORF1蛋白的动态表达模式相关。
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A search for reverse transcriptase-coding sequences reveals new non-LTR retrotransposons in the genome of Drosophila melanogaster.对逆转录酶编码序列的搜索揭示了黑腹果蝇基因组中的新型非LTR逆转座子。
Genome Biol. 2000;1(6):RESEARCH0012. doi: 10.1186/gb-2000-1-6-research0012. Epub 2000 Dec 4.
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Retrotransposition of a marked Drosophila line-like I element in cells in culture.
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8
Long-distance interactions between regulatory elements are suppressed at the end of a terminally deficient chromosome in Drosophila melanogaster.在黑腹果蝇中，调控元件之间的长距离相互作用在末端缺失染色体的末端受到抑制。
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Chimeric RNA transposition intermediates of the I factor produce precise retrotransposed copies.I因子的嵌合RNA转座中间体产生精确的逆转座拷贝。
Nucleic Acids Res. 2002 Aug 1;30(15):3387-94. doi: 10.1093/nar/gkf456.

引用本文的文献

1
In vivo RNA localization of I factor, a non-LTR retrotransposon, requires a cis-acting signal in ORF2 and ORF1 protein.非长末端重复逆转座子I因子的体内RNA定位需要开放阅读框2（ORF2）和开放阅读框1（ORF1）蛋白中的顺式作用信号。
Nucleic Acids Res. 2005 Feb 1;33(2):776-85. doi: 10.1093/nar/gki221. Print 2005.
2
Gene silencing triggered by non-LTR retrotransposons in the female germline of Drosophila melanogaster.果蝇雌性生殖系中由非长末端重复序列逆转座子引发的基因沉默。
Genetics. 2003 Jun;164(2):521-31. doi: 10.1093/genetics/164.2.521.
3
Artificial and epigenetic regulation of the I factor, a nonviral retrotransposon of Drosophila melanogaster.果蝇非病毒逆转座子I因子的人工调控和表观遗传调控
Genetics. 2000 Dec;156(4):1867-78. doi: 10.1093/genetics/156.4.1867.
4
New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.关于果蝇中含有ORF1的转基因对I因子活性的同源依赖性沉默的新见解。
Genetics. 2000 Nov;156(3):1147-55. doi: 10.1093/genetics/156.3.1147.
5
High-frequency retrotransposition of a marked I factor in Drosophila melanogaster correlates with a dynamic expression pattern of the ORF1 protein in the cytoplasm of oocytes.果蝇中一个标记的I因子的高频逆转座与卵母细胞细胞质中ORF1蛋白的动态表达模式相关。
Genetics. 1999 Feb;151(2):761-71. doi: 10.1093/genetics/151.2.761.

本文引用的文献

1
High genetic instability of heterochromatin after transposition of the LINE-like I factor in Drosophila melanogaster.果蝇中类LINE元件I因子转座后异染色质的高遗传不稳定性
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8052-7. doi: 10.1073/pnas.94.15.8052.
2
Many human L1 elements are capable of retrotransposition.许多人类L1元件能够进行逆转座。
Nat Genet. 1997 May;16(1):37-43. doi: 10.1038/ng0597-37.
3
High frequency retrotransposition in cultured mammalian cells.培养的哺乳动物细胞中的高频逆转座
Cell. 1996 Nov 29;87(5):917-27. doi: 10.1016/s0092-8674(00)81998-4.
4
Human L1 retrotransposon encodes a conserved endonuclease required for retrotransposition.人类L1逆转座子编码一种逆转座所需的保守内切核酸酶。
Cell. 1996 Nov 29;87(5):905-16. doi: 10.1016/s0092-8674(00)81997-2.
5
Control of expression of the I factor, a LINE-like transposable element in Drosophila melanogaster.果蝇中类LINE转座因子I因子表达的调控
EMBO J. 1996 Jun 17;15(12):3174-81.
6
mRNA retroposition in human cells: processed pseudogene formation.人类细胞中的mRNA反转座：加工假基因的形成。
EMBO J. 1995 Dec 15;14(24):6333-8. doi: 10.1002/j.1460-2075.1995.tb00324.x.
7
Germ-line expression of the I factor, a functional LINE from the fruit fly Drosophila melanogaster, is positively regulated by reactivity, a peculiar cellular state.I因子是果蝇黑腹果蝇中的一种功能性长散在核元件（LINE），其种系表达受反应性（一种特殊的细胞状态）的正向调控。
Mol Gen Genet. 1993 Aug;240(2):277-85. doi: 10.1007/BF00277067.
8
Generation of processed pseudogenes in murine cells.小鼠细胞中加工假基因的产生。
EMBO J. 1993 Apr;12(4):1487-97. doi: 10.1002/j.1460-2075.1993.tb05792.x.
9
The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression.I因子的5'非翻译区是黑腹果蝇中一种长散在核元件样逆转座子，它包含一个内部启动子和调控表达的序列。
Mol Cell Biol. 1993 Feb;13(2):1042-50. doi: 10.1128/mcb.13.2.1042-1050.1993.
10
Type I repressors of P element mobility.P 因子移动性的 I 型阻遏物。
Genetics. 1993 Sep;135(1):81-95. doi: 10.1093/genetics/135.1.81.

当以反式提供开放阅读框2（ORF2）时，果蝇中的一个基因标记I元件可以被激活。

A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.

作者信息

Busseau I, Malinsky S, Balakireva M, Chaboissier M C, Teninges D, Bucheton A

机构信息

Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.

出版信息

Genetics. 1998 Jan;148(1):267-75. doi: 10.1093/genetics/148.1.267.

DOI:10.1093/genetics/148.1.267
PMID:9475738
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1459780/
Abstract

I factors in Drosophila melanogaster are non-LTR retrotransposons similar to mammalian LINEs. They transpose at very high frequencies in the germ line of SF females resulting from crosses between reactive females, devoid of active I factors, and inducer males, containing active I factors. The vermilion marked IviP2 element was designed to allow easy phenotypical screening for retrotransposition events. It is deleted in ORF2 and therefore cannot produce reverse transcriptase. IviP2 can be mobilized at very low frequencies by actively transposing I factors in the germ line of SF females. This paper shows that IviP2 can be mobilized more efficiently in the germ line of strongly reactive females in the absence of active I factors, when it is trans-complemented by the product of ORF2 synthesized from the hsp70 heat-shock promoter. This represents a promising step toward the use of marked I elements to study retrotransposition and as tools for mutagenesis.

摘要

果蝇中的I因子是非LTR反转录转座子，类似于哺乳动物的LINEs。它们在无活性I因子的反应性雌性与含有活性I因子的诱导性雄性杂交产生的SF雌性的生殖系中以非常高的频率转座。朱砂标记的IviP2元件旨在便于对反转录转座事件进行表型筛选。它在ORF2中缺失，因此不能产生逆转录酶。IviP2可以通过SF雌性生殖系中活跃转座的I因子以非常低的频率被激活。本文表明，当由hsp70热休克启动子合成的ORF2产物进行反式互补时，IviP2在无活性I因子的强反应性雌性的生殖系中可以更有效地被激活。这代表了朝着使用标记的I元件来研究反转录转座并作为诱变工具迈出的有希望的一步。