一项用于鉴定果蝇眼睛发育中sina信号通路组成成分的遗传筛选。
A genetic screen to identify components of the sina signaling pathway in Drosophila eye development.
作者信息
Neufeld T P, Tang A H, Rubin G M
机构信息
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
出版信息
Genetics. 1998 Jan;148(1):277-86. doi: 10.1093/genetics/148.1.277.
Abstract
Specification of the R7 photoreceptor cell in the developing Drosophila eye requires the seven in absentia (sina) gene. We demonstrate that ectopic expression of sina in all cells behind the morphogenetic furrow disrupts normal eye development during pupation, resulting in a severely disorganized adult eye. Earlier events of cell fate specification appear unaffected. A genetic screen for dominant enhancers and suppressors of this phenotype identified mutations in a number of genes required for normal eye development, including UbcD1, which encodes a ubiquitin conjugating enzyme; SR3-4a, a gene previously implicated in signaling downstream of Ras1; and a Drosophila homolog of the Sin3A transcriptional repressor.
摘要
在发育中的果蝇眼睛中，R7光感受器细胞的特化需要无七（sina）基因。我们证明，在形态发生沟后的所有细胞中异位表达sina会在化蛹期间破坏正常的眼睛发育，导致成虫眼睛严重紊乱。早期的细胞命运特化事件似乎未受影响。对该表型的显性增强子和抑制子进行的遗传筛选鉴定出了一些正常眼睛发育所需基因中的突变，包括编码泛素结合酶的UbcD1；SR3-4a，一个先前与Ras1下游信号传导有关的基因；以及Sin3A转录抑制因子的果蝇同源物。
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Ras1 signaling and transcriptional competence in the R7 cell of Drosophila.果蝇R7细胞中的Ras1信号传导与转录能力
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A screen for genes that function downstream of Ras1 during Drosophila eye development.在果蝇眼睛发育过程中对Ras1下游发挥作用的基因进行筛选。
Genetics. 1996 May;143(1):315-29. doi: 10.1093/genetics/143.1.315.
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Proc Natl Acad Sci U S A. 1996 May 14;93(10):5025-30. doi: 10.1073/pnas.93.10.5025.
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