Leonard J P, Beckers C
Clin Chim Acta. 1976 Jul 1;70(1):119-22. doi: 10.1016/0009-8981(76)90012-7.
Various factors that influence the measurement of serum folic acid-binding protein (FABP) by radioassay were examined. Varying the protein concentration and the composition of the charcoal suspension can increase or reduce the value of the blank, i.e. the estimate of FABP. Moreover, it was found that the value of the FABP depends on the quantity of folic acid in the incubation medium. Reproducible results can only be obtained with rigorously defined conditions of concentration of the different reactants.
研究了影响通过放射分析测定血清叶酸结合蛋白(FABP)的各种因素。改变蛋白质浓度和活性炭悬浮液的组成可以增加或降低空白值,即FABP的估计值。此外,还发现FABP的值取决于孵育介质中叶酸的量。只有在严格定义不同反应物浓度的条件下才能获得可重复的结果。