核p53在膀胱癌、前列腺癌和肾癌中的过表达。
Nuclear p53 overexpression in bladder, prostate, and renal carcinomas.
作者信息
Sinik Z, Alkibay T, Ataoglu O, Biri H, Sözen S, Deniz N, Karaoglan U, Bozkirli I
机构信息
Department of Urology, School of Medicine, Gazi University, Ankara, Turkey.
出版信息
Int J Urol. 1997 Nov;4(6):546-51. doi: 10.1111/j.1442-2042.1997.tb00306.x.
BACKGROUND
The aim of this study was to examine nuclear p53 overexpression in transitional cell carcinoma of the bladder, adenocarcinoma of the prostate, and renal cell carcinoma.
METHODS
Forty-four pathologic specimens from 39 bladder cancer patients, 41 prostatic adenocarcinoma, and 39 renal cell carcinoma specimens were analyzed immunohistochemically with D07 monoclonal antibody to detect the expression of the mutant p53 gene. Overexpression was said to occur when the number of positively-stained tumor nuclei were > or = 10% in each specimen. p53 overexpression was correlated with the clinical and histopathological features of these cancers.
RESULTS
Nuclear p53 overexpression occurred in 18.2% of transitional cell bladder cancer specimens, 12.2% of prostate cancer specimens, and 17.9% of renal cell cancer specimens. Statistical analyses showed that grade, vascular invasion, and necrosis in bladder cancer, a high Gleason score in prostate cancer, and the 1-year mortality rate in renal cancer were significantly related with p53 nuclear overexpression (P < 0.05).
CONCLUSION
Using the D07 monoclonal antibody, nuclear p53 overexpression is relatively uncommon in urologic malignancies, and moderately correlates with several histopathological and clinical features of urologic malignancies.
背景
本研究旨在检测膀胱移行细胞癌、前列腺腺癌和肾细胞癌中细胞核p53的过表达情况。
方法
采用D07单克隆抗体对39例膀胱癌患者的44份病理标本、41份前列腺腺癌标本和39份肾细胞癌标本进行免疫组化分析，以检测突变型p53基因的表达。当每个标本中阳性染色的肿瘤细胞核数量≥10%时，即认为发生了过表达。p53过表达与这些癌症的临床和组织病理学特征相关。
结果
细胞核p53过表达在膀胱移行细胞癌标本中占18.2%，在前列腺癌标本中占12.2%，在肾细胞癌标本中占17.9%。统计分析表明，膀胱癌的分级、血管侵犯和坏死、前列腺癌的高Gleason评分以及肾癌的1年死亡率与p53细胞核过表达显著相关（P<0.05）。
结论
使用D07单克隆抗体检测，细胞核p53过表达在泌尿系统恶性肿瘤中相对少见，且与泌尿系统恶性肿瘤的一些组织病理学和临床特征中度相关。
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