Meedeniya A C, Brookes S J, Hennig G W, Costa M
Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100, Adelaide, South Australia, Australia.
Cell Tissue Res. 1998 Mar;291(3):375-84. doi: 10.1007/s004410051007.
Retrograde tracing, combined with immunohistochemistry, was used to study the projections of 5-hydroxytryptamine (5-HT)-accumulating neurones within the ileum of the guinea-pig, with confocal microscopy being used to characterise further their morphology. Two classes of neurones in the myenteric plexus, capable of taking up 5-HT or analogues, were distinguished. One class had Dogiel type I morphology with lamellar dendrites, was located on the edge or in the middle of ganglia and lacked immunoreactivity for somatostatin (SOM). The other class had smooth ovoid cell bodies with multiple filamentous dendrites and a single axon and represented a subset of the SOM-immunoreactive interneurones in the myenteric plexus. Varicosities immunoreactive for 5-HT alone, 5-HT/SOM or SOM alone were present in the myenteric ganglia. Both classes of 5-HT-accumulating neurones had long aboral projections within the myenteric plexus (up to 100 mm long) and to the submucous plexus and probably function as descending interneurones.
逆行追踪结合免疫组织化学方法，用于研究豚鼠回肠内5-羟色胺（5-HT）积聚神经元的投射，并用共聚焦显微镜进一步表征其形态。在肌间神经丛中区分出两类能够摄取5-HT或类似物的神经元。一类具有Dogiel I型形态，有板层状树突，位于神经节边缘或中间，对生长抑素（SOM）无免疫反应性。另一类具有光滑的卵圆形细胞体，有多个丝状树突和单个轴突，代表肌间神经丛中SOM免疫反应性中间神经元的一个子集。在肌间神经节中存在仅对5-HT、5-HT/SOM或仅对SOM有免疫反应性的曲张体。两类5-HT积聚神经元在肌间神经丛内都有长的向肛投射（长达100毫米）并投射到黏膜下神经丛，可能起下行中间神经元的作用。
