Song Z M, Brookes S J, Costa M
Department of Physiology, Centre for Neuroscience, The Flinders University of South Australia, GPO Box 2100, Adelaide 5001, Australia.
Cell Tissue Res. 1996 Jul;285(1):149-56. doi: 10.1007/s004410050630.
The projections of myenteric neurons within the myenteric plexus of the guinea-pig small intestine were established using retrograde tracing in organotypic culture. Three days after applying the fluorescent dye DiI to a single internodal strand in the myenteric plexus, 500-1000 nerve cell bodies were labelled. Of these, 77% were located oral to the application site, 15% were located anally and 7% were located within 1 mm of this site. Three major morphological types of neurons could be distinguished. Dogiel type I neurons had lamellar dendrites and single axons, Dogiel type II neurons had large smooth cell bodies and several long processes, and filamentous neurons had smooth ovoid cell bodies, single axons and several filamentous dendrites. Dogiel type I, II and filamentous neurons accounted for 54.6%, 38% and 7.4% of all filled cells, respectively. Labelled nerve cell bodies were present up to 13 mm aboral to the DiI application site; all neurons more than 2 mm aboral had Dogiel type I features. On the oral side, Dogiel type I neurons were found up to 110 mm, Dogiel type II neurons up to 100 mm and filamentous neurons up to 80 mm. Neurons with 2 mm oral or aboral to the DiI application site were located up to 7 mm circumferentially and were mainly Dogiel type II cells. This work revealed remarkable polarity within the myenteric plexus, with a significant prevalence of myenteric neurons projecting anally for longer distances than those projecting orally. These long pathways are probably involved in the coordination of intestinal motility.
运用器官型培养中的逆行追踪法，确定了豚鼠小肠肌间神经丛内肌间神经元的投射。在将荧光染料DiI应用于肌间神经丛的单个节间束三天后，有500 - 1000个神经细胞体被标记。其中，77%位于应用部位的口侧，15%位于肛侧，7%位于该部位1毫米范围内。可区分出三种主要形态类型的神经元。多极I型神经元有板层状树突和单根轴突，多极II型神经元有大的光滑细胞体和几条长突起，丝状神经元有光滑的卵圆形细胞体、单根轴突和几条丝状树突。多极I型、II型和丝状神经元分别占所有被填充细胞的54.6%、38%和7.4%。在DiI应用部位肛侧13毫米处均有标记的神经细胞体；所有距肛侧超过2毫米的神经元都有多极I型特征。在口侧，多极I型神经元在距应用部位110毫米处被发现，多极II型神经元在距应用部位100毫米处被发现，丝状神经元在距应用部位80毫米处被发现。在DiI应用部位口侧或肛侧2毫米范围内的神经元，在圆周方向上可达7毫米，主要是多极II型细胞。这项研究揭示了肌间神经丛内显著的极性，肌间神经元向肛侧投射的距离明显长于向口侧投射的距离。这些长通路可能参与肠道运动的协调。
