Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada; Department of Immunology and Internal Medicine section of Gastroenterology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
FEBS Lett. 2014 Apr 17;588(8):1480-90. doi: 10.1016/j.febslet.2014.02.002. Epub 2014 Feb 15.
Neurons in the enteric nervous system utilize numerous neurotransmitters to orchestrate rhythmic gut smooth muscle contractions. We examined whether electrical synapses formed by gap junctions containing connexin36 also contribute to communication between enteric neurons in mouse colon. Spontaneous contractility properties and responses to electrical field stimulation and cholinergic agonist were altered in gut from connexin36 knockout vs. wild-type mice. Immunofluorescence revealed punctate labelling of connexin36 that was localized at appositions between somata of enteric neurons immunopositive for the enzyme nitric oxide synthase. There is indication for a possible functional role of gap junctions between inhibitory nitrergic enteric neurons.
肠神经系统中的神经元利用多种神经递质来协调肠道平滑肌的有节奏收缩。我们研究了由包含连接蛋白 36 的缝隙连接形成的电突触是否也有助于小鼠结肠中的肠神经元之间的通讯。与野生型相比，缝隙连接蛋白 36 敲除小鼠的肠道自发性收缩特性以及对电刺激和胆碱能激动剂的反应发生了改变。免疫荧光显示连接蛋白 36 的点状标记，该标记定位于免疫阳性的一氧化氮合酶的肠神经元体之间的贴合处。这表明抑制性氮能肠神经元之间的缝隙连接可能具有功能作用。
