唾液或血清对变形链球菌和白色念珠菌在羟基磷灰石珠上定殖的影响。

The effect of saliva or serum on Streptococcus mutans and Candida albicans colonization of hydroxylapatite beads.

作者信息

Nikawa H, Hamada T, Yamashiro H, Murata H, Subiwahjudi A

机构信息

Department of Prosthetic Dentistry, Hiroshima University School of Dentistry, Japan.

出版信息

J Dent. 1998 Jan;26(1):31-7. doi: 10.1016/s0300-5712(96)00076-0.

DOI:10.1016/s0300-5712(96)00076-0

PMID:9479923

Abstract

OBJECTIVE

Several recent reports imply the possibility of cariogenicity of denture plaque containing Candida albicans. Hence the purpose of this study was to investigate the effects of salivary and serum pellicles on C. albicans and Streptococcus mutans colonization on hydroxylapatite beads.

METHODS

The colonization of three isolates of C. albicans and two isolates of S. mutans was examined by the use of a bioluminescent adenosine triphosphate (ATP) assay based on the firefly luciferase-luciferin system.

RESULTS

In the preliminary study, a good correlation was observed between the cell number and ATP amount of each isolate tested, and the results yielded a level of significance (P < 0.001; Student's t-test), confirming the validity of this method. When the relative ATP content of the 48 h colonization of both isolates of S. mutans were compared, a saliva pellicle was significantly more effective in promoting bacterial colonization than either uncoated or serum pellicle (ANOVA; P < 0.01). In contrast, in the case of colonization of C. albicans isolates, a serum pellicle was significantly more effective in promoting the colonization of C. albicans GDH 18 and GDH 19, than both uncoated specimens and saliva pellicle (ANOVA; P < 0.01). Similar trends were observed with C. albicans GDH 16, though significant differences were not observed (ANOVA; P > 0.05).

CONCLUSION

The results suggest that the mechanism involved in fungal colonization on hydroxylapatite (HAP) should be different from that of mutans streptococci.

摘要

目的

近期的几份报告表明含白色念珠菌的义齿菌斑具有致龋性。因此，本研究旨在探讨唾液和血清薄膜对白色念珠菌和变形链球菌在羟基磷灰石珠粒上定植的影响。

方法

使用基于萤火虫荧光素酶 - 荧光素系统的生物发光三磷酸腺苷（ATP）测定法，检测三株白色念珠菌和两株变形链球菌的定植情况。

结果

在初步研究中，观察到所测试的每种分离株的细胞数量与ATP含量之间具有良好的相关性，结果具有显著性水平（P < 0.001；Student氏t检验），证实了该方法的有效性。当比较两株变形链球菌48小时定植的相对ATP含量时，唾液薄膜在促进细菌定植方面比未包被或血清薄膜显著更有效（方差分析；P < 0.01）。相比之下，在白色念珠菌分离株定植的情况下，血清薄膜在促进白色念珠菌GDH 18和GDH 19的定植方面比未包被标本和唾液薄膜显著更有效（方差分析；P < 0.01）。白色念珠菌GDH 16也观察到类似趋势，尽管未观察到显著差异（方差分析；P > 0.05）。