Molecular Identification of in Endodontic Retreatment Cases by SYBR Green I Real-time Polymerase Chain Reaction and its Association with Endodontic Symptoms.

STATEMENT OF THE PROBLEM

Recent microbiological studies have expressed ever-increasing concerns about as a causal factor in the failure of endodontic treatments. Real-time quantitative polymerase chain reaction (qPCR), including the SYBR Green I system, is a technique in which a fluorescent dye is incorporated into the double-stranded DNA that is produced during DNA polymerase activity.

PURPOSE

This study aimed to determine the relative prevalence of in root canals of retreatment cases and its association with endodontic symptoms.

MATERIALS AND METHOD

In the present cross-sectional/analytical study fifty subjects were selected. Clinical features and radiographic status of the teeth were also evaluated. After access cavity preparation, the retrieved material and dentinal chips removed from the root canal were transferred into 1.5-mL microtubes, followed by storage at -20ºC until used for DNA extraction. A DNeasy Tissue Kit was used to extract DNA using the DNeasy protocol for animal tissues. Master Plus SYBR Green I (Jena Bioscience, Germany) was used in a Rotor-gene Real-time PCR System for real-time PCR. The relationship between the presence of and the clinical and radiographic features were analyzed using McNemar's test.

RESULTS

There was a significant relationship between the radiographic findings in endodontically treated teeth and the presence of . However, there was no significant relationship between the presence of and any of the clinical symptoms.

CONCLUSION

In spite of the limitations of this study, we concluded that was associated with root canal infections in endodontic retreatment cases, but there was no relationship between root canal infections and the clinical symptoms.