Eichler H, Klinge U, Kretschmer V
Abteilung für Transfusionsmedizin und Gerinnungsphysiologie, Universitätsklinikum Marburg, Deutschland.
Beitr Infusionsther Transfusionsmed. 1994;32:149-52.
The gel centrifugation test ID Microtyping was compared with the column agglutination test BioVue for antibody (AB) screening by testing serum samples of 3,000 patients under controlled conditions: enzyme test (ET; Bromelin) and LISS indirect antiglobulin test (IAT), both incubated for 15 min at 37 degrees C. From a total of 64 ABs, more relevant or possibly relevant ABs were detected by ID than by the BioVue system (63 vs. 56). ABs only detected in ID were: anti-D 3, -Jk(a), E 1, -Le(b)1, -e 1, -C 1, -E,S 1. One anti-Le(b) was only detected by BioVue. The calculated sensitivity was 98.4% for ID and 87.5% for BioVue. Furthermore, this study showed a lower frequency of unspecific reactions or reactions due to irrelevant cold ABs in the ID system (ID: 2.3%, BioVue: 4.0%, specificity 97.7 vs. 96.2%).
