Eichler H, Kretschmer V
Abteilung für Transfusionsmedizin und Gerinnungsphysiologie, Universitätsklinikum Marburg, Deutschland.
Beitr Infusionsther Transfusionsmed. 1994;32:156-8.
We studied 97 samples of patients being positive in the autocontrol of the indirect antiglobulin test (IAT) in the gel system (DiaMed). In 83.2%, retesting with monospecific anti-IgG serum gave also positive results, due to a specific phenomenon caused, for example, by drug-specific antibodies (AB), warm auto-AB or allo-AB. In contrast, only 52.9% of the samples retested by the standard tube technique with polyspecific antiglobulin serum reacted positive. Only in 6 patients slightly increased cold agglutinins could be detected. None of the investigated patients showed any clinical or laboratory signs of hemolysis except one with pernicious anemia. We conclude that positive results of the autocontrol in the gel IAT should be confirmed by an additional DAT in the tube technique. If this second test shows a negative result, transfusions can take place without any restrictions.
我们研究了97例在凝胶系统（DiaMed）间接抗球蛋白试验（IAT）自身对照中呈阳性的患者样本。在83.2%的样本中，用单特异性抗IgG血清重新检测也得到阳性结果，这是由例如药物特异性抗体（AB）、温自身抗体或同种异体抗体引起的特定现象所致。相比之下，用多特异性抗球蛋白血清通过标准试管技术重新检测的样本中，只有52.9%呈阳性反应。仅在6例患者中检测到冷凝集素略有升高。除1例患有恶性贫血的患者外，所调查的患者均未表现出任何溶血的临床或实验室迹象。我们得出结论，凝胶IAT自身对照的阳性结果应以试管技术进行额外的直接抗球蛋白试验（DAT）来确认。如果第二次检测结果为阴性，则输血可不受任何限制地进行。
