Richter E, Matthes G, Tofoté U, Rath E, Takahashi T A, Hosoda M, Sekiguchi S
Institut für Transfusionsmedizin, Medizinische Fakultät (Charité), Humboldt-Universität zu Berlin.
Beitr Infusionsther Transfusionsmed. 1994;32:26-8.
Whole blood (WB) and SAGM-resuspended red cell concentrates (RCC) were leucocyte-depleted 1 h after donation by filtration using BF 4 (PALL), Sepacell RS 200 (Asahi Med.) and Bio R01 Plus (Biotrans). We compared the handling of the filter system, focusing routine blood bank practice, filtration efficiency [residual white cell count; flow cytometry (Ortho Cytoron) and Nageotte chamber] and the loss of red cells. Spontaneous filling was found to be an advantage of the Bio R01 Plus system, whereas the BPF 4 and the RS 200 have to be filled by pressure. The filtrations were of similar duration, even those of WB and resuspended RCC. Red cell loss was found to be 10-15% on RCC higher than on WB (7-10%) and varied between the filters used: BPF 4 < Bio R01 Plus < RS 200. The residual white cell count on WB was 1.2-5.8 x 10(6) (RS 200 < Bio R01 Plus < BPF 4) and on RCC was 0.5-3.0 x 10(5) (RS 200 < BPF 4 < Bio R01 Plus). The filters used are highly effective (4-log depletion on RCC) even on fresh WB and are therefore potential in-line filters.
全血（WB）和SAGM重悬红细胞浓缩物（RCC）在采集后1小时通过使用BF 4（颇尔公司）、Sepacell RS 200（旭化成医疗株式会社）和Bio R01 Plus（生物转化公司）进行过滤以去除白细胞。我们比较了过滤系统的操作，重点关注常规血库操作、过滤效率[残留白细胞计数；流式细胞术（Ortho Cytoron）和纳盖特计数板]以及红细胞损失情况。发现Bio R01 Plus系统的一个优点是能够自发填充，而BPF 4和RS 200则必须通过压力填充。过滤持续时间相似，即使是全血和重悬红细胞浓缩物的过滤也是如此。发现红细胞损失在红细胞浓缩物上比在全血上高10% - 15%（全血为7% - 10%），并且在所使用的过滤器之间有所不同：BPF 4 < Bio R01 Plus < RS 200。全血上的残留白细胞计数为1.2 - 5.8×10⁶（RS 200 < Bio R01 Plus < BPF 4），红细胞浓缩物上的残留白细胞计数为0.5 - 3.0×10⁵（RS 200 < BPF 4 < Bio R01 Plus）。所使用的过滤器即使对新鲜全血也非常有效（对红细胞浓缩物有4个对数级的去除效果），因此是潜在的在线过滤器。
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