[通过在线过滤进行白细胞去除]

[Leukocyte depletion by in-line-filtration].

作者信息

Krandick E, Vornwald A, Gossrau E

机构信息

Blutspendedienst im Deutschen Roten Kreuz Berlin gGmbH, Deutschland.

出版信息

Beitr Infusionsther Transfusionsmed. 1994;32:6-8.

PMID:9480158

Abstract

Leukocyte depletion was tested in 30 red cell concentrates (RCC) using a new 450-ml triple U blood-bag system (Optipac, Baxter) with an integrated polyester filter (Sepacell R 2000). Centrifugation at 3,600 rpm was well tolerated by all filters. RCC were filtered 48 h after preparation (mean time 25-30 min). Filtration loss of red blood cells was about 7%. Prefiltration leukocyte concentration averaged 3 x 10(9) leukocytes/l (Coulter). After filtration 6 x 10(5) leukocytes/l (median) were counted in the Nageotte chamber (NC). 1.5 x 10(5)/RCC leukozytes (WBC) per filtered RCC were calculated (median). 29/30 (97%) RCC contained less than 5 x 10(6) WBC, in 4 cases no WBC were detectable in the NC, but 6.62 x 10(6) WBC remained in 1 RCC. The rate of hemolysis (8/30) averaged 0.4% on day 42. Handling was easy, allowing routine use. Quality controls are necessary.

摘要

使用带有一体式聚酯过滤器（Sepacell R 2000）的新型450毫升三联U型血袋系统（Optipac，百特公司），对30份红细胞浓缩液（RCC）进行白细胞去除测试。所有过滤器对3600转/分钟的离心均耐受良好。RCC在制备后48小时进行过滤（平均时间25 - 30分钟）。红细胞的过滤损失约为7%。过滤前白细胞浓度平均为3×10⁹白细胞/升（库尔特计数法）。在纳盖奥特计数室（NC）中，过滤后计数的白细胞中位数为6×10⁵白细胞/升。计算得出每份过滤后的RCC中白细胞（WBC）含量为1.5×10⁵/RCC（中位数）。29/30（97%）的RCC中白细胞含量少于5×10⁶，在4份样本中，NC中未检测到白细胞，但有1份RCC中仍残留6.62×10⁶白细胞。在第42天，溶血率（8/30）平均为0.4%。操作简便，可常规使用。质量控制是必要的。