Zingsem J, Serke S, Weisbach V, Zimmermann R, Zeiler T, Kampe D, Kunhardt O, Eckstein R
Abteilung für Transfusionsmedizin, Friedrich-Alexander-Universität Erlangen, Deutschland.
Beitr Infusionsther Transfusionsmed. 1994;32:405-7.
Peripheral blood-derived haematopoietic stem cells (PBSC) are used as an alternative to bone marrow stem cells for autologous transplantation. One of the most important prerequisites for successful PBSC separation is the precise determination of the optimal separation days. As we previously demonstrated that neither PLT counts nor WBC counts in the peripheral blood (PB) are of predictive value for the amount of colony-forming cells (CFC) in the patients' PB, we started a daily monitoring of CD-34-positive cells to determine the beginning of the separation series. In addition to routine cell counts and CFU testing we assessed the number of CD 34+ in the PBSC concentrates to ascertain the number of separations needed for each patient. Due to the strong correlation of CD 34+ cells to CFC it is possible to predict the number of CFC collected within 2 h after finishing the PBSC separation and to calculate the efficiency of the separation for quality control.
外周血来源的造血干细胞（PBSC）被用作自体移植中骨髓干细胞的替代物。成功分离PBSC的最重要前提之一是精确确定最佳分离天数。正如我们之前所证明的，外周血（PB）中的血小板计数和白细胞计数对于患者PB中集落形成细胞（CFC）的数量均无预测价值，因此我们开始每日监测CD-34阳性细胞以确定分离系列的开始时间。除了常规细胞计数和CFU检测外，我们还评估了PBSC浓缩物中CD 34+的数量，以确定每位患者所需的分离次数。由于CD 34+细胞与CFC之间存在强相关性，因此在完成PBSC分离后2小时内就可以预测收集到的CFC数量，并计算分离效率以进行质量控制。
