Zingsem J, Zeiler T, Weisbach V, Musch R, Heuft H G, Siegert W, Eckstein R
Abteilung Innere Medizin, Universitätsklinikum Rudolf Virchow, Standort Charlottenburg, Freie Universität Berlin.
Beitr Infusionsther. 1990;26:197-9.
For some years, there has been an increasing success in transplanting PBSC instead of autologous bone marrow in patients suffering from leukemic diseases. In healthy cytapheresis donors, we achieved peripheral blood mononuclear cell (PBM) recoveries of 74%, using a discontinuous flow separation technique (Haemonetics V50, Lymphosurge). At the moment, we have collected PBSC from 4 patients (3 AML, 1 ALL) in 28 cytapheresis procedures ranging from 12.9% to 80.1% (mean: 40.1%), whereas stem cell recoveries, defined by CFU-GM, were significantly better (mean: 66%). At present, one of these patients has been transplanted with PBSC alone, receiving 2.11 x 10(8) PBM/kg with 2.8 x 10(4) CFU-GM/kg. His posttransplantation cytopenia was shorter compared to other patients undergoing autologous bone marrow transplantation. The period of disease-free survival is now more than eight months while he is completely reintegrated into social and working life.
多年来，在白血病患者中，移植外周血干细胞（PBSC）而非自体骨髓取得了越来越大的成功。在健康的血细胞分离术供者中，我们使用间断流分离技术（Haemonetics V50，Lymphosurge）实现了74%的外周血单个核细胞（PBM）回收率。目前，我们已通过28次血细胞分离术从4例患者（3例急性髓系白血病、1例急性淋巴细胞白血病）中采集了PBSC，回收率在12.9%至80.1%之间（平均：40.1%），而由粒-巨噬细胞集落形成单位（CFU-GM）定义的干细胞回收率明显更高（平均：66%）。目前，其中1例患者仅接受了PBSC移植，每千克体重接受2.11×10⁸个PBM和2.8×10⁴个CFU-GM/kg。与其他接受自体骨髓移植的患者相比，他移植后的血细胞减少期更短。无病生存期现已超过8个月，此时他已完全重新融入社会和工作生活。
