Dittmann J, Riggert J, Wieding J U, Simson G, Köhler M
Abteilung Transfusionsmedizin, Universitätskliniken, Göttingen, Deutschland.
Beitr Infusionsther Transfusionsmed. 1994;32:422-7.
Membrane glycoprotein (GP) expression on platelets changes during preparation of concentrates and storage. Recent studies proved that the increased expression of the GMP-140 (CD 62), a GP appearing on platelets after activation, correlates with the in vivo recovery of transfused platelets. We therefore investigated the expression of this and three other GP (GP IIb/IIIa, GP, Ib, GP 53), detected by flow cytometry, in normal controls and in platelet concentrates prepared by four different methods (apheresis, preparation from buffy coat, from pooled buffy coat and platelet-rich plasma) after preparation and during storage. Furthermore we investigated the influence of filtration, radiation and washing platelet concentrates on the expression of GP. The results can be summarized as follows: (1) The mean surface fluorescence (MF) is a better parameter for the evaluation of preparation and storage lesions than the total number of positive cells. (2) The mean fluorescence of GP Ib in platelet concentrates is generally lower than in normal controls and decreases continuously during storage; there is a small loss of GP Ib through washing and filtration. (3) GP IIb/IIIa fluctuates during preparation and storage, therefore it is not suitable for evaluation of quality. (4) The percentage of CD 62 positive cells and their mean fluorescence is higher in platelet concentrates than in normal controls and increases during storage continuously. There is a weak correlation of CD 62 with CD 63. (5) The changes of GP in apheresis concentrates and concentrates obtained from whole blood are similar after preparation and during storage.
在浓缩血小板制备和储存过程中，血小板膜糖蛋白（GP）的表达会发生变化。最近的研究证明，激活后出现在血小板上的糖蛋白GMP - 140（CD 62）表达增加与输注血小板的体内回收率相关。因此，我们通过流式细胞术检测了正常对照以及用四种不同方法（单采、从 Buffy 层制备、从混合 Buffy 层和富血小板血浆制备）制备的血小板浓缩物在制备后和储存期间这种糖蛋白以及其他三种糖蛋白（GP IIb/IIIa、GP Ib、GP 53）的表达情况。此外，我们还研究了过滤、辐射和洗涤血小板浓缩物对 GP 表达的影响。结果总结如下：（1）平均表面荧光（MF）比阳性细胞总数更适合作为评估制备和储存损伤的参数。（2）血小板浓缩物中 GP Ib 的平均荧光通常低于正常对照，且在储存期间持续下降；洗涤和过滤会导致 GP Ib 有少量损失。（3）GP IIb/IIIa 在制备和储存过程中波动，因此不适合用于评估质量。（4）血小板浓缩物中 CD 62 阳性细胞的百分比及其平均荧光高于正常对照，且在储存期间持续增加。CD 62 与 CD 63 之间存在弱相关性。（5）单采浓缩物和从全血获得的浓缩物在制备后和储存期间 GP 的变化相似。
