Schoofs L, Hamdaoui A, Devreese B, Van Beeumen J, De Loof A
Zoological Institute, Katholieke Universiteit Leuven, Belgium.
Biochem Biophys Res Commun. 1998 Feb 13;243(2):390-4. doi: 10.1006/bbrc.1998.8104.
A novel, highly hydrophobic, glycine- and proline-rich peptide was characterized in the ovary of the desert locust, Schistocerca gregaria. The peptide was detected as one of the major peaks in a chromatographic separation of an acidic methanolic extract of 50 mature ovaries. Electrospray mass spectrometry yielded a molecular mass of 6305 Da. The partial amino acid sequence as determined by Edman degradation based automated microsequencing is as follows: Ala-Tyr-Pro-Ala-His-Gln-Gly-Tyr- Pro-Ala-His-Val-Gly-Tyr-Ala-Arg-Val-Gly-Tyr-Gly- Gly-Tyr-Pro-Ser-Tyr-Gly-Tyr-Pro-Ala. Four amino acids (Gly, Pro, Ala, and Tyr) account for more than 80% of the composition of this sequence. Gly-Tyr-Pro is the most important repetitive motif. Ala-Tyr-Pro, Gly-Tyr-Gly and Gly-Tyr-Ala occur as variations of this motif. The novel glycine- and proline-rich insect peptide displays structural characteristics similar to those of a new class of glycine- and proline-rich proteins (GPRP) that have recently been identified in Arabidopsis thaliana (thale cress) and Daucus carota (carrot). The GPRP of A. thaliana contains the same repetitive motifs (except for Ala-Tyr-Pro), the Gly-Tyr-Pro motif also being the most abundant.
在沙漠蝗（Schistocerca gregaria）的卵巢中鉴定出一种新型的、高度疏水的、富含甘氨酸和脯氨酸的肽。该肽在50个成熟卵巢的酸性甲醇提取物的色谱分离中被检测为主要峰之一。电喷雾质谱法测得其分子量为6305 Da。通过基于埃德曼降解的自动微量测序法确定的部分氨基酸序列如下：Ala-Tyr-Pro-Ala-His-Gln-Gly-Tyr-Pro-Ala-His-Val-Gly-Tyr-Ala-Arg-Val-Gly-Tyr-Gly-Gly-Tyr-Pro-Ser-Tyr-Gly-Tyr-Pro-Ala。四种氨基酸（甘氨酸、脯氨酸、丙氨酸和酪氨酸）占该序列组成的80%以上。Gly-Tyr-Pro是最重要的重复基序。Ala-Tyr-Pro、Gly-Tyr-Gly和Gly-Tyr-Ala是该基序的变体。这种新型的富含甘氨酸和脯氨酸的昆虫肽显示出与最近在拟南芥（thale cress）和胡萝卜（Daucus carota）中鉴定出的一类新型富含甘氨酸和脯氨酸的蛋白质（GPRP）相似的结构特征。拟南芥的GPRP包含相同的重复基序（除了Ala-Tyr-Pro），Gly-Tyr-Pro基序也是最丰富的。
