Shankar S, Mohapatra B, Suri A
Genes and Proteins Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Biochem Biophys Res Commun. 1998 Feb 13;243(2):561-5. doi: 10.1006/bbrc.1997.7943.
A novel gene coding for a human sperm surface protein (HSS) has been cloned from a human testis cDNA library. The 2.5 kb cDNA (hss) contains five direct repeats, three mirror repeats, three possible stem loop structures and three unique palindromic motifs located at functionally relevant positions. Open reading frame encodes a protein of 766 amino acids. The deduced protein analysis revealed a NH2 terminus extracellular domain having a leucine zipper (LZ) motif, a transmembrane helical domain and a cytoplasmic domain at the COOH terminus. Tissue specific expression studies demonstrated the presence of hss transcript only in testis. Cell type expression of hss mRNA was observed only on the round spermatids suggesting haploid germ cell expression.
从人睾丸cDNA文库中克隆出了一个编码人精子表面蛋白（HSS）的新基因。这个2.5kb的cDNA（hss）包含五个直接重复序列、三个镜像重复序列、三个可能的茎环结构以及位于功能相关位置的三个独特回文基序。开放阅读框编码一个由766个氨基酸组成的蛋白质。推导的蛋白质分析显示，其氨基末端细胞外结构域具有亮氨酸拉链（LZ）基序，一个跨膜螺旋结构域以及位于羧基末端的细胞质结构域。组织特异性表达研究表明，hss转录本仅存在于睾丸中。仅在圆形精子细胞上观察到hss mRNA的细胞类型表达，提示单倍体生殖细胞表达。
