Mohapatra B, Verma S, Shankar S, Suri A
Genes and Proteins Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Biochem Biophys Res Commun. 1998 Mar 17;244(2):540-5. doi: 10.1006/bbrc.1998.8079.
We have cloned a human testis specific gene (hi), which encodes for a protein having regional homologies to the domain of A-kinase anchoring proteins. Open reading frame encodes a protein of 860 amino acids. The predicted protein has a molecular weight 95.8 kD and contains 32 cysteine residues and 34 potential phosphorylation sites. The deduced protein analysis revealed an eukaryotic secretory signal with a potential cleavage site. Northern blot analysis detected a single transcript of approximately 3.0 kb in testis and not in any other somatic tissues. Expression of hi transcript was observed only in round spermatids indicating post meiotic haploid gene expression.
我们克隆了一个人类睾丸特异性基因（hi），它编码一种与A激酶锚定蛋白结构域具有区域同源性的蛋白质。开放阅读框编码一个含860个氨基酸的蛋白质。预测的蛋白质分子量为95.8 kD，含有32个半胱氨酸残基和34个潜在的磷酸化位点。推导的蛋白质分析显示有一个具有潜在切割位点的真核分泌信号。Northern印迹分析在睾丸中检测到一个约3.0 kb的单一转录本，而在任何其他体细胞组织中均未检测到。仅在圆形精子细胞中观察到hi转录本的表达，表明减数分裂后单倍体基因表达。
