Ide N, Hirao K, Hata Y, Takeuchi M, Irie M, Yao I, Deguchi M, Toyoda A, Nishioka H, Mizoguchi A, Takai Y
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, Kobe, Japan.
Biochem Biophys Res Commun. 1998 Feb 13;243(2):634-8. doi: 10.1006/bbrc.1998.8142.
In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 encodes a small protein with one PDZ domain. The lin-10 product has no homology to known proteins. Here, we have cloned a rat homologue of the lin-10 product and characterized it. Rat lin-10 is ubiquitously expressed in various rat tissues and distributed in both the cytosol and membrane fractions. In brain, however, rat lin-10 is distributed only in the membrane fraction and enriched in the synaptic plasma membrane and postsynaptic density fractions. These results suggest that rat lin-10 is involved at least in synaptic functions in brain.
在秀丽隐杆线虫中，外阴诱导由由lin-3、let-23和let-60产物组成的酪氨酸激酶受体/Ras信号转导途径介导。除了这些基因产物外，lin-2、lin-7和lin-10产物也参与该途径。Lin-2编码一种MAGUK，lin-7编码一种具有一个PDZ结构域的小蛋白。lin-10产物与已知蛋白质无同源性。在这里，我们克隆了lin-10产物的大鼠同源物并对其进行了表征。大鼠lin-10在大鼠的各种组织中普遍表达，分布于细胞质和膜组分中。然而，在大脑中，大鼠lin-10仅分布于膜组分中，并富集于突触质膜和突触后致密组分中。这些结果表明，大鼠lin-10至少参与大脑中的突触功能。
