通过在酿酒酵母中的异源表达鉴定秀丽隐杆线虫Δ6-脂肪酸去饱和酶
Identification of a Caenorhabditis elegans Delta6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae.
作者信息
Napier J A, Hey S J, Lacey D J, Shewry P R
机构信息
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.
出版信息
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):611-4. doi: 10.1042/bj3300611.
Abstract
We identified a cDNA expressed sequence tag from an animal (the nematode worm Caenorhabditis elegans) that showed weak similarity to a higher-plant microsomal Delta6-desaturase. A full-length cDNA clone was isolated and expressed in the yeast Saccharomyces cerevisiae. This demonstrated that the protein encoded by the C. elegans cDNA was that of a fatty acid Delta6-desaturase, as determined by the accumulation of gamma-linolenic acid. The C. elegans Delta6-desaturase contained an N-terminalcytochrome b5 domain, indicating that it had a similar structure to that of the higher-plant Delta6-desaturase. The C. elegans Delta6-desaturase mapped to cosmid W08D2, a region of chromosome III. This is the first example of a Delta6-desaturase isolated from an animal and also the first example of an animal desaturase containing a cytochrome b5 domain.
摘要
我们从一种动物（线虫秀丽隐杆线虫）中鉴定出一个cDNA表达序列标签，它与高等植物微粒体Δ6-去饱和酶有微弱的相似性。分离出一个全长cDNA克隆并在酿酒酵母中表达。这表明，由秀丽隐杆线虫cDNA编码的蛋白质是一种脂肪酸Δ6-去饱和酶，这是通过γ-亚麻酸的积累确定的。秀丽隐杆线虫的Δ6-去饱和酶含有一个N端细胞色素b5结构域，表明它与高等植物Δ6-去饱和酶具有相似的结构。秀丽隐杆线虫的Δ6-去饱和酶定位于粘粒W08D2，即第三条染色体的一个区域。这是从动物中分离出的Δ6-去饱和酶的第一个例子，也是含有细胞色素b5结构域的动物去饱和酶的第一个例子。
相似文献
1
Identification of a Caenorhabditis elegans Delta6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae.通过在酿酒酵母中的异源表达鉴定秀丽隐杆线虫Δ6-脂肪酸去饱和酶
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):611-4. doi: 10.1042/bj3300611.
2
Functional identification of a fatty acid delta5 desaturase gene from Caenorhabditis elegans.秀丽隐杆线虫脂肪酸Δ5去饱和酶基因的功能鉴定
FEBS Lett. 1998 Nov 20;439(3):215-8. doi: 10.1016/s0014-5793(98)01385-4.
3
Delta6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Gene cloning and its heterologous expression in a fungus, Aspergillus.来自一种产生花生四烯酸的被孢霉属真菌的Δ6-脂肪酸去饱和酶。基因克隆及其在真菌曲霉中的异源表达。
Gene. 1999 Oct 1;238(2):445-53. doi: 10.1016/s0378-1119(99)00359-5.
4
[Cloning and heterologous expression of a novel delta6 -desaturase gene from Rhizopus arrhizus NK030037].[来自少根根霉NK030037的新型Δ6-去饱和酶基因的克隆与异源表达]
Yi Chuan Xue Bao. 2004 Jul;31(7):740-9.
5
A vertebrate fatty acid desaturase with Delta 5 and Delta 6 activities.一种具有Δ5和Δ6活性的脊椎动物脂肪酸去饱和酶。
Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14304-9. doi: 10.1073/pnas.251516598. Epub 2001 Nov 27.
6
[Cloning and expression of delta6-desaturase gene from Thamnidium elegans in Saccharomyces cerevisiae].[雅致枝霉Δ6-脂肪酸去饱和酶基因在酿酒酵母中的克隆与表达]
Wei Sheng Wu Xue Bao. 2006 Feb;46(1):74-9.
7
A bifunctional delta-fatty acyl acetylenase/desaturase from the moss Ceratodon purpureus. A new member of the cytochrome b5 superfamily.来自紫萼藓（Ceratodon purpureus）的一种双功能δ-脂肪酸酰基乙炔酶/去饱和酶。细胞色素b5超家族的一个新成员。
Eur J Biochem. 2000 Jun;267(12):3801-11. doi: 10.1046/j.1432-1327.2000.01418.x.
8
Cloning of delta12- and delta6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae.来自高山被孢霉的Δ12-和Δ6-去饱和酶的克隆及在酿酒酵母中γ-亚麻酸的重组生产。
Lipids. 1999 Jul;34(7):649-59. doi: 10.1007/s11745-999-0410-8.
9
Molecular cloning and functional characterization of a Δ6-fatty acid desaturase gene from Rhizopus oryzae.从米根霉中克隆和功能表征 Δ6-脂肪酸去饱和酶基因。
J Basic Microbiol. 2013 Sep;53(9):773-7. doi: 10.1002/jobm.201200189. Epub 2012 Sep 7.
10
Identification and characterization of a novel delta6-fatty acid desaturase gene from Rhizopus nigricans.从黑根霉中鉴定和表征一种新型的 delta6-脂肪酸去饱和酶基因。
Mol Biol Rep. 2009 Nov;36(8):2291-7. doi: 10.1007/s11033-009-9447-0. Epub 2009 Jan 17.
引用本文的文献
1
Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster.在果蝇中，通过线虫脂肪酸去饱和酶和延伸酶生物合成 n-3 多不饱和脂肪酸。
Transgenic Res. 2023 Oct;32(5):411-421. doi: 10.1007/s11248-023-00363-9. Epub 2023 Aug 24.
2
Acyl-Lipid Δ-Desaturase May Act as a First FAD in Cyanobacteria.酰基辅酶 A-脂 Δ-去饱和酶可能在蓝细菌中充当第一黄素腺嘌呤二核苷酸。
Biomolecules. 2022 Dec 1;12(12):1795. doi: 10.3390/biom12121795.
3
NADPH-Cytochrome P450 Reductase Mediates the Fatty Acid Desaturation of ω3 and ω6 Desaturases from .NADPH-细胞色素P450还原酶介导来自……的ω3和ω6去饱和酶的脂肪酸去饱和作用 。
Curr Issues Mol Biol. 2022 Apr 22;44(5):1828-1837. doi: 10.3390/cimb44050125.
4
Multifaceted regulation and functions of fatty acid desaturase 2 in human cancers.脂肪酸去饱和酶2在人类癌症中的多方面调控及功能
Am J Cancer Res. 2020 Dec 1;10(12):4098-4111. eCollection 2020.
5
Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use.Δ6 脂肪酸去饱和酶在多不饱和脂肪酸生物合成中的作用：进化、功能与底物特异性及生物技术应用的见解。
Appl Microbiol Biotechnol. 2020 Dec;104(23):9947-9963. doi: 10.1007/s00253-020-10958-5. Epub 2020 Oct 23.
6
Juniperonic Acid Biosynthesis is Essential in Lacking Δ6 Desaturase () and Generates New ω-3 Endocannabinoids.金雀花酸生物合成在缺乏 Δ6 去饱和酶 () 的情况下是必需的，并产生新的 ω-3 内源性大麻素。
Cells. 2020 Sep 19;9(9):2127. doi: 10.3390/cells9092127.
7
Lipid and Carbohydrate Metabolism in .脂质与碳水化合物代谢于…… （你提供的原文不完整，后面应该还有具体内容）
Genetics. 2017 Oct;207(2):413-446. doi: 10.1534/genetics.117.300106.
8
Yeast-Based High-Throughput Screens to Identify Novel Compounds Active against Brugia malayi.基于酵母的高通量筛选以鉴定对马来布鲁线虫有效的新型化合物。
PLoS Negl Trop Dis. 2016 Jan 26;10(1):e0004401. doi: 10.1371/journal.pntd.0004401. eCollection 2016 Jan.
9
The codon-optimized Δ(6)-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis.腐霉菌密码子优化的Δ(6)-去饱和酶基因作为工程化n3/n6多不饱和脂肪酸生物合成的有力工具。
BMC Biotechnol. 2015 Sep 15;15:82. doi: 10.1186/s12896-015-0200-6.
10
Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis.拟南芥中ω-3多不饱和脂肪酸的异源重组
Biomed Res Int. 2015;2015:768478. doi: 10.1155/2015/768478. Epub 2015 Aug 3.
本文引用的文献
1
Dissecting desaturation: plants prove advantageous.解剖性去饱和：植物显示出优势。
Trends Cell Biol. 1996 Apr;6(4):148-53. doi: 10.1016/0962-8924(96)10002-7.
2
A new class of cytochrome b5 fusion proteins.一类新型的细胞色素b5融合蛋白。
Biochem J. 1997 Dec 1;328 ( Pt 2)(Pt 2):717-8.
3
The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis.
Biochemistry. 1997 Jun 10;36(23):6960-7. doi: 10.1021/bi970091l.
4
Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.含有N端细胞色素b5结构域的琉璃苣去饱和酶cDNA的表达导致转基因烟草中高水平的δ6-去饱和脂肪酸积累。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4211-6. doi: 10.1073/pnas.94.8.4211.
5
Mössbauer studies of alkane omega-hydroxylase: evidence for a diiron cluster in an integral-membrane enzyme.烷烃ω-羟化酶的穆斯堡尔研究：膜整合酶中二铁簇的证据
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2981-6. doi: 10.1073/pnas.94.7.2981.
6
Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis.通过在拟南芥中的异源表达鉴定一种动物ω-3脂肪酸去饱和酶。
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1142-7. doi: 10.1073/pnas.94.4.1142.
7
Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.生长温度对自由生活线虫秀丽隐杆线虫脂肪酸组成的影响。
Lipids. 1996 Nov;31(11):1173-8. doi: 10.1007/BF02524292.
8
A novel cytochrome b5-like domain is linked to the carboxyl terminus of the Saccharomyces cerevisiae delta-9 fatty acid desaturase.一个新的细胞色素b5样结构域与酿酒酵母Δ9脂肪酸去饱和酶的羧基末端相连。
J Biol Chem. 1995 Dec 15;270(50):29766-72. doi: 10.1074/jbc.270.50.29766.
9
Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase.八个组氨酸残基在一种膜相关铁酶——硬脂酰辅酶A去饱和酶中对催化作用至关重要，并且在烷烃羟化酶和二甲苯单加氧酶中保守存在。
Biochemistry. 1994 Nov 1;33(43):12787-94. doi: 10.1021/bi00209a009.
10
Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis.拟南芥FAD2基因编码对多不饱和脂质合成至关重要的酶。
Plant Cell. 1994 Jan;6(1):147-58. doi: 10.1105/tpc.6.1.147.
Zotero 插件