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本文引用的文献

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Trends Cell Biol. 1996 Apr;6(4):148-53. doi: 10.1016/0962-8924(96)10002-7.
2
A new class of cytochrome b5 fusion proteins.一类新型的细胞色素b5融合蛋白。
Biochem J. 1997 Dec 1;328 ( Pt 2)(Pt 2):717-8.
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The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis.
Biochemistry. 1997 Jun 10;36(23):6960-7. doi: 10.1021/bi970091l.
4
Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.含有N端细胞色素b5结构域的琉璃苣去饱和酶cDNA的表达导致转基因烟草中高水平的δ6-去饱和脂肪酸积累。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4211-6. doi: 10.1073/pnas.94.8.4211.
5
Mössbauer studies of alkane omega-hydroxylase: evidence for a diiron cluster in an integral-membrane enzyme.烷烃ω-羟化酶的穆斯堡尔研究：膜整合酶中二铁簇的证据
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2981-6. doi: 10.1073/pnas.94.7.2981.
6
Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis.通过在拟南芥中的异源表达鉴定一种动物ω-3脂肪酸去饱和酶。
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1142-7. doi: 10.1073/pnas.94.4.1142.
7
Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.生长温度对自由生活线虫秀丽隐杆线虫脂肪酸组成的影响。
Lipids. 1996 Nov;31(11):1173-8. doi: 10.1007/BF02524292.
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A novel cytochrome b5-like domain is linked to the carboxyl terminus of the Saccharomyces cerevisiae delta-9 fatty acid desaturase.一个新的细胞色素b5样结构域与酿酒酵母Δ9脂肪酸去饱和酶的羧基末端相连。
J Biol Chem. 1995 Dec 15;270(50):29766-72. doi: 10.1074/jbc.270.50.29766.
9
Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase.八个组氨酸残基在一种膜相关铁酶——硬脂酰辅酶A去饱和酶中对催化作用至关重要，并且在烷烃羟化酶和二甲苯单加氧酶中保守存在。
Biochemistry. 1994 Nov 1;33(43):12787-94. doi: 10.1021/bi00209a009.
10
Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis.拟南芥FAD2基因编码对多不饱和脂质合成至关重要的酶。
Plant Cell. 1994 Jan;6(1):147-58. doi: 10.1105/tpc.6.1.147.

通过在酿酒酵母中的异源表达鉴定秀丽隐杆线虫Δ6-脂肪酸去饱和酶

Identification of a Caenorhabditis elegans Delta6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae.

作者信息

Napier J A, Hey S J, Lacey D J, Shewry P R

机构信息

IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.

出版信息

Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):611-4. doi: 10.1042/bj3300611.

DOI:10.1042/bj3300611
PMID:9480865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1219180/
Abstract

We identified a cDNA expressed sequence tag from an animal (the nematode worm Caenorhabditis elegans) that showed weak similarity to a higher-plant microsomal Delta6-desaturase. A full-length cDNA clone was isolated and expressed in the yeast Saccharomyces cerevisiae. This demonstrated that the protein encoded by the C. elegans cDNA was that of a fatty acid Delta6-desaturase, as determined by the accumulation of gamma-linolenic acid. The C. elegans Delta6-desaturase contained an N-terminalcytochrome b5 domain, indicating that it had a similar structure to that of the higher-plant Delta6-desaturase. The C. elegans Delta6-desaturase mapped to cosmid W08D2, a region of chromosome III. This is the first example of a Delta6-desaturase isolated from an animal and also the first example of an animal desaturase containing a cytochrome b5 domain.

摘要

我们从一种动物（线虫秀丽隐杆线虫）中鉴定出一个cDNA表达序列标签，它与高等植物微粒体Δ6-去饱和酶有微弱的相似性。分离出一个全长cDNA克隆并在酿酒酵母中表达。这表明，由秀丽隐杆线虫cDNA编码的蛋白质是一种脂肪酸Δ6-去饱和酶，这是通过γ-亚麻酸的积累确定的。秀丽隐杆线虫的Δ6-去饱和酶含有一个N端细胞色素b5结构域，表明它与高等植物Δ6-去饱和酶具有相似的结构。秀丽隐杆线虫的Δ6-去饱和酶定位于粘粒W08D2，即第三条染色体的一个区域。这是从动物中分离出的Δ6-去饱和酶的第一个例子，也是含有细胞色素b5结构域的动物去饱和酶的第一个例子。