Speer C A, Dubey J P
Department of Veterinary Molecular Biology, Montana State University, Bozeman 59717-3610, USA.
Parasitology. 1998 Jan;116 ( Pt 1):35-42. doi: 10.1017/s0031182097001959.
Transmission electron microscopy was used to study Toxoplasma gondii infections in the small intestines of Swiss-Webster mice at 2-48 h post-feeding of oocysts (p.f.). Sporozoites passed through intestinal epithelial cells (enterocytes and goblet cells) and infected all cells except red blood cells in the lamina propria. Parasites in intestinal epithelial cells or in cells in the lamina propria were located within a single type of parasitophorous vacuole, which contained exocytosed electron-dense material and well-developed tubulovesicular membranous networks. Sporozoites did not infect intraepithelial lymphocytes (IELs), but at 48 h p.f. IELs had become infected with tachyzoites arising from those that had developed in the lamina propria. At 48 h p.f., the lamina propria contained numerous tachyzoites, much cellular debris, and few intact cells. The intestinal epithelium exhibited limited cytopathological changes except for villar fusion, slight vacuolation, and cell separation at the bases of enterocytes.
利用透射电子显微镜研究了瑞士韦伯斯特小鼠在喂食卵囊后2至48小时小肠内的弓形虫感染情况。子孢子穿过肠上皮细胞（肠细胞和杯状细胞），感染了固有层中除红细胞以外的所有细胞。肠上皮细胞或固有层细胞中的寄生虫位于单一类型的寄生泡内，该寄生泡含有胞吐的电子致密物质和发育良好的管状泡状膜网络。子孢子不感染上皮内淋巴细胞（IEL），但在喂食后48小时，IEL被固有层中发育而来的速殖子感染。在喂食后48小时，固有层中有大量速殖子、许多细胞碎片和少量完整细胞。除了绒毛融合、轻微空泡化和肠细胞基部的细胞分离外，肠上皮表现出有限的细胞病理学变化。
