Shikimi T, Gonda T, Takahashi M, Kaneto H, Okunishi H, Takaori S
Department of Pharmacology, Shimane Medical University, Enya, Izumo, Japan.
Neurochem Res. 1998 Jan;23(1):69-72. doi: 10.1023/a:1022453504527.
Effects of ischemia on the content of a ulinastatin (UT)-like substance in the murine cerebral cortex and hippocampus were studied. At 24 h post-ischemia, a significant (p < 0.05) decrease in the content of UT-like substance in the hippocampus but not the cerebral cortex and a concurrent increase in the activity of micro-calpain were observed. In in vitro experiments, a decrease was registered in the content of UT-like substance in the hippocampus in the presence of calcium. This decrease was inhibited by both EDTA and calpastatin treatments. These results implicate the destruction of UT-like substance by micro-calpain in the ischemic hippocampus.
研究了缺血对小鼠大脑皮层和海马中类乌司他丁（UT）物质含量的影响。缺血后24小时，观察到海马中类UT物质含量显著（p < 0.05）降低，但大脑皮层中未出现此现象，同时微钙蛋白酶活性增加。在体外实验中，存在钙的情况下，海马中类UT物质含量降低。乙二胺四乙酸（EDTA）和钙蛋白酶抑制蛋白处理均能抑制这种降低。这些结果表明，缺血海马中的微钙蛋白酶可破坏类UT物质。
