扫描流式细胞仪流动比色皿及光学装置的新设计。

A new design of the flow cuvette and optical set-up for the scanning flow cytometer.

作者信息

Soini J T, Chernyshev A V, Hänninen P E, Soini E, Maltsev V P

机构信息

Department of Medical Physics and Chemistry, University of Turku, Finland.

出版信息

Cytometry. 1998 Feb 1;31(2):78-84. doi: 10.1002/(sici)1097-0320(19980201)31:2<78::aid-cyto2>3.0.co;2-e.

DOI:10.1002/(sici)1097-0320(19980201)31:2<78::aid-cyto2>3.0.co;2-e

PMID:9482276

Abstract

We introduce a new design for the optical cuvette and a new optical lay-out for the Scanning Flow Cytometer (SFC) that permits measurement of the angular dependency of the scattered light from individual moving particles. The improved optical scheme of the SFC allows measurement of the angular scattering pattern of individual particles at polar angles from 10 degrees to 120 degrees with integration at azimuthal angles from 0 degrees to 360 degrees and with angular resolution of better than 0.5 degrees. The performance of the SFC is demonstrated using certified polystyrene particles as reference material The aim of this work is to develop a flow cytometer, which, by recording the entire light scattering pattern of individual biological particles, would provide more information about the particle structure than the ordinary wide angle, forward and side scattering concepts.

摘要

我们介绍了一种用于光学比色皿的新设计以及一种用于扫描流式细胞仪（SFC）的新光学布局，该布局允许测量来自单个移动粒子的散射光的角度依赖性。改进后的SFC光学方案能够测量单个粒子在10度至120度极角范围内的角散射图案，方位角范围为0度至360度，角分辨率优于0.5度。使用经过认证的聚苯乙烯颗粒作为参考材料展示了SFC的性能。这项工作的目的是开发一种流式细胞仪，通过记录单个生物粒子的整个光散射图案，能够比普通的广角、前向和侧向散射概念提供更多关于粒子结构的信息。