Heyneman R A
Arch Int Physiol Biochim. 1979 Dec;87(5):925-34. doi: 10.3109/13813457909070540.
The extent of the cyanide-resistent oxidative burst of polymorpho-nuclear leukocytes after stimulation with cytochalasin E was shown to depend markedly on the osmolarity of the cell-suspension medium. With granulocyte concentrations up to 2 X 10(6) cells/ml, optimal oxygen consumption and releases of H2O2 and superoxide anions were reached at 180 mOsmol and 2 X 10(-5) M cytochalasin E. After removal of unbound activator, the cellular oxidative activity remained unaltered and continued to depend on the used osmotic conditions. It is proposed that binding of cytochalasin to the plasma membrane induces an irreversible activation of the oxidative system, whereas the resulting metabolic activity depends on conformational changes in the plasma membrane.
在用细胞松弛素E刺激后，多形核白细胞对氰化物抵抗性氧化爆发的程度被证明明显取决于细胞悬浮培养基的渗透压。当粒细胞浓度高达2×10⁶个细胞/毫升时，在180毫渗摩尔和2×10⁻⁵ M细胞松弛素E的条件下可达到最佳耗氧量以及过氧化氢和超氧阴离子的释放量。去除未结合的激活剂后，细胞氧化活性保持不变，并继续取决于所使用的渗透条件。有人提出，细胞松弛素与质膜的结合会诱导氧化系统的不可逆激活，而由此产生的代谢活性则取决于质膜的构象变化。
