Kuralay F, Akarca U S, Ozütemiz A O, Kutay F, Batur Y
Dokuz Eylül University School of Medicine, Department of Biochemistry, Izmir, Turkey.
J Toxicol Environ Health A. 1998 Feb 6;53(3):223-9. doi: 10.1080/009841098159358.
It has been reported that fish oil protects the rat liver against acetaminophen (APAP) induced toxicity; however, this finding is controversial. The present study was undertaken to investigate the effects of fish oil-enriched diet on APAP-induced liver injury in Wistar rats. Rats were fed a diet supplemented with either 8% fish oil or 8% corn oil, or standard rat feed for 6 wk. After an overnight fast, rats in each group were given either 2 g/kg APAP or saline orally. Our findings showed that APAP increased serum alanine aminotransferase (ALT) and that this rise was potentiated in the presence of dietary fat. Further fish oil ingestion increased the glutathione (GSH) content in rat liver; however, this was not effective in protecting liver from APAP-induced toxicity. Data suggest that GSH may be necessary to detoxify APAP metabolites, which are known to induce hepatotoxicity but are increased by dietary fat.
据报道，鱼油可保护大鼠肝脏免受对乙酰氨基酚（APAP）诱导的毒性作用；然而，这一发现存在争议。本研究旨在探讨富含鱼油的饮食对Wistar大鼠APAP诱导的肝损伤的影响。将大鼠分别喂食补充8%鱼油或8%玉米油的饲料，或标准大鼠饲料，持续6周。禁食过夜后，每组大鼠口服给予2 g/kg APAP或生理盐水。我们的研究结果表明，APAP会使血清丙氨酸转氨酶（ALT）升高，并且在存在膳食脂肪的情况下这种升高会增强。进一步摄入鱼油会增加大鼠肝脏中的谷胱甘肽（GSH）含量；然而，这对保护肝脏免受APAP诱导的毒性无效。数据表明，GSH可能是使APAP代谢产物解毒所必需的，已知这些代谢产物会诱导肝毒性，但会因膳食脂肪而增加。
