Spatz S J, Maes R K
Department of Microbiology, Michigan State University, East Lansing 48824.
Virology. 1993 Nov;197(1):125-36. doi: 10.1006/viro.1993.1573.
Feline herpesvirus 1 (FHV-1) is an important viral pathogen of cats. Like other alphaherpesviruses, FHV-1 contains a HSV-1 glycoprotein B (gB) homolog. In this study, monospecific antisera to HSV-1 gB reacted with three FHV-1 proteins (100, 64, and 58 kDa) present in virion lysates by immunoprecipitation and immunoblot analyses. Reduced stringency hybridization experiments using a HSV-1 gB probe localized the FHV-1 gB gene to a 9.6-kb Sa/l fragment in the unique long region of the genome. Northern blot analyses further localized the entire coding region within a 3.3-kb SacI fragment. The nucleotide sequence of this fragment was determined and two overlapping open reading frames (ORFs) encoding gB and ICP 18.5 were predicted. The amino acid sequence of the 2829 bp gB ORF was shown to have a high degree of homology with gB analogs of HSV-1, EHV-1, BHV-1, EHV-4, and especially PRV. Two unique characteristics of gB of FHV-1 were the unusually long signal sequence of 73 residues and two potential internal cleavage sites, RTRRS and RSRRS. An evolutionary tree based on gB homologs from 12 alphaherpesviruses suggests that feline herpesvirus-1 evolved along similar lines as the varicelloviruses, pseudorabies virus, bovine herpesvirus type 1, and equine herpesvirus types 1 and 4. The gB gene of FHV-1 was expressed in vaccinia virus (WR). This recombinant induced fairly high titers of virus neutralizing antibodies in rabbits. In Western blot analyses with potassium tartrate-purified virions, a 60-kDa polypeptide reacted with the rabbit antisera.
猫疱疹病毒1型（FHV-1）是猫的一种重要病毒病原体。与其他α疱疹病毒一样，FHV-1含有一种单纯疱疹病毒1型糖蛋白B（gB）同源物。在本研究中，针对单纯疱疹病毒1型gB的单特异性抗血清通过免疫沉淀和免疫印迹分析与病毒粒子裂解物中存在的三种FHV-1蛋白（100、64和58 kDa）发生反应。使用单纯疱疹病毒1型gB探针进行的低严谨度杂交实验将FHV-1 gB基因定位到基因组独特长区域中的一个9.6 kb Sa/l片段。Northern印迹分析进一步将整个编码区域定位在一个3.3 kb SacI片段内。测定了该片段的核苷酸序列，并预测了两个重叠的开放阅读框（ORF），分别编码gB和ICP 18.5。2829 bp的gB ORF的氨基酸序列显示与单纯疱疹病毒1型、马疱疹病毒1型、牛疱疹病毒1型、马疱疹病毒4型，尤其是伪狂犬病病毒的gB类似物具有高度同源性。FHV-1 gB的两个独特特征是异常长的73个残基的信号序列和两个潜在的内部切割位点，RTRRS和RSRRS。基于12种α疱疹病毒gB同源物构建的进化树表明，猫疱疹病毒1型与水痘病毒、伪狂犬病病毒、牛疱疹病毒1型以及马疱疹病毒1型和4型沿着相似的路线进化。FHV-1的gB基因在痘苗病毒（WR）中表达。这种重组体在兔中诱导出相当高滴度的病毒中和抗体。在用酒石酸钾纯化的病毒粒子进行的Western印迹分析中，一种60 kDa的多肽与兔抗血清发生反应。
