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几种菜豆（Phaseolus vulgaris）核转运RNA基因的结构与表达：与tRNA导入植物线粒体过程的相关性

Structure and expression of several bean (Phaseolus vulgaris) nuclear transfer RNA genes: relevance to the process of tRNA import into plant mitochondria.

作者信息

Ramamonjisoa D, Kauffmann S, Choisne N, Maréchal-Drouard L, Green G, Wintz H, Small I, Dietrich A

机构信息

Institut de Biologie Moléculaire des Plantes, UPR A0406 du CNRS, Université Louis Pasteur, Strasbourg, France.

出版信息

Plant Mol Biol. 1998 Mar;36(4):613-25. doi: 10.1023/a:1005972023506.

DOI:10.1023/a:1005972023506
PMID:9484456
Abstract

Bean nuclear genes for tRNA(Pro), tRNA(Thr) and tRNA(Leu) were isolated. Expression of the tRNA(Pro) genes was demonstrated in vivo and sequence analysis suggested amplification of the tRNA(Pro) gene copy number through duplication of a gene cluster at the same locus of the bean genome. The two tRNA(Thr) genes isolated were actively transcribed and their transcripts processed in a HeLa cell system. In vivo expression tests of these genes and aminoacylation assays of the corresponding in vitro transcripts showed the presence of identity determinants in the anticodon of plant tRNA(Thr). The tRNA(Leu) gene was not expressed due to deviation from the consensus in the internal B-box promoter. The same sequence deviation also prevented aminoacylation of the corresponding in vitro transcript. This tRNA(Leu) however exists in plants and is synthesized from another gene with a consensus B-box promoter. Plant mitochondria import from the cytosol a number of nucleus-encoded tRNAs, including tRNA(Leu) and tRNA(Thr). From the available sequence data, we could not identify any conserved structural motif characteristic for the nucleus-encoded tRNAs imported into plant mitochondria, either in the tRNAs, or in the gene flanking sequences. These results suggest that recognition of tRNAs for import is idiosyncratic and likely to depend on protein/RNA interactions that are specific to each tRNA or each isoacceptor group.

摘要

分离出了豆类中编码tRNA（Pro）、tRNA（Thr）和tRNA（Leu）的核基因。在体内证实了tRNA（Pro）基因的表达，序列分析表明，通过豆类基因组同一基因座处基因簇的重复，tRNA（Pro）基因拷贝数得以扩增。分离出的两个tRNA（Thr）基因在HeLa细胞系统中被积极转录并对其转录本进行了加工。这些基因的体内表达测试以及相应体外转录本的氨酰化测定表明，植物tRNA（Thr）的反密码子中存在身份决定因素。由于内部B盒启动子与共有序列存在偏差，tRNA（Leu）基因未表达。同样的序列偏差也阻止了相应体外转录本的氨酰化。然而，这种tRNA（Leu）存在于植物中，是由另一个具有共有B盒启动子的基因合成的。植物线粒体从细胞质中导入许多核编码的tRNA，包括tRNA（Leu）和tRNA（Thr）。根据现有的序列数据，我们无法在tRNA或基因侧翼序列中识别出导入植物线粒体的核编码tRNA所特有的任何保守结构基序。这些结果表明，tRNA导入的识别是特异的，可能取决于每个tRNA或每个同功受体组特有的蛋白质/RNA相互作用。

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1
Structure and expression of several bean (Phaseolus vulgaris) nuclear transfer RNA genes: relevance to the process of tRNA import into plant mitochondria.几种菜豆（Phaseolus vulgaris）核转运RNA基因的结构与表达：与tRNA导入植物线粒体过程的相关性
Plant Mol Biol. 1998 Mar;36(4):613-25. doi: 10.1023/a:1005972023506.
2
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The anticodon and the D-domain sequences are essential determinants for plant cytosolic tRNA(Val) import into mitochondria.反密码子和D结构域序列是植物胞质tRNA（Val）导入线粒体的关键决定因素。
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Editing and import: strategies for providing plant mitochondria with a complete set of functional transfer RNAs.编辑与导入：为植物线粒体提供一整套功能性转运RNA的策略
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In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?正常或诱变的异源转运RNA在转基因植物线粒体中的体内导入：影响线粒体基因表达的新途径？
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In vitro import of a nuclearly encoded tRNA into mitochondria of Solanum tuberosum.核编码的tRNA体外导入马铃薯线粒体。

本文引用的文献

1
The sequences of two nuclear genes and a pseudogene for tRNA(Pro) from the higher plant Phaseolus vulgaris.高等植物菜豆（Phaseolus vulgaris）的 tRNA(Pro)的两个核基因和一个假基因的序列。
Plant Mol Biol. 1986 May;7(3):207-12. doi: 10.1007/BF00021332.
2
APhaseolus vulgaris mitochondrial tRNA(Leu) is identical to its cytoplasmic counterpart: sequencing andin vivo transcription of the gene corresponding to the cytoplasmic tRNA(Leu.).菜豆线粒体 tRNA（亮氨酸）与其细胞质对应物完全相同：对应于细胞质 tRNA（亮氨酸）的基因的测序和体内转录。
Plant Mol Biol. 1987 Jan;10(1):13-9. doi: 10.1007/BF00014182.
3
Effects of 5' flanking sequences and changes in the 5' internal control region on the transcription of rice tRNA % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaqcKbay-haafaqabe% GabaaabaGaae4raiaabYgacaqG5baabaGaae4raiaaboeacaqGdbaa% aaaa!3CC7!\[\begin{array}{*{20}c} {{\text{Gly}}} \\ {{\text{GCC}}} \\ \end{array} \].
Mol Cell Biol. 2003 Jun;23(11):4000-12. doi: 10.1128/MCB.23.11.4000-4012.2003.
4
Higher plant mitochondria.高等植物线粒体
Plant Cell. 1999 Apr;11(4):571-86. doi: 10.1105/tpc.11.4.571.
5'侧翼序列和 5'内部调控区变化对水稻 tRNA % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaqcKbay-haafaqabe% GabaaabaGaae4raiaabYgacaqG5baabaGaae4raiaaboeacaqGdbaa% aaaa!3CC7!\[\begin{array}{*{20}c} {{\text{Gly}}} \\ {{\text{GCC}}} \\ \end{array} \]的转录影响。
Plant Mol Biol. 1988 Sep;11(5):575-83. doi: 10.1007/BF00017457.
4
Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.在植物细胞和转基因植物中萤火虫荧光素酶基因的瞬时和稳定表达。
Science. 1986 Nov 14;234(4778):856-9. doi: 10.1126/science.234.4778.856.
5
Implication of 5'-flanking sequence elements in expression of a plant tRNA(Leu) gene.5'-侧翼序列元件对植物tRNA（Leu）基因表达的影响
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6
Transactivation of a target gene using a suppressor tRNA in transgenic tobacco plants.在转基因烟草植株中使用抑制性tRNA对靶基因进行反式激活。
Plant J. 1997 Mar;11(3):597-604. doi: 10.1046/j.1365-313x.1997.11030597.x.
7
Accumulation of nuclear-encoded tRNA(Thr) (AGU) in mitochondria of the liverwort Marchantia polymorpha.地钱（Marchantia polymorpha）肝脏线粒体中核编码的tRNA（Thr）（AGU）的积累。
Biochim Biophys Acta. 1997 Feb 28;1350(3):262-6. doi: 10.1016/s0167-4781(96)00239-4.
8
A single base change prevents import of cytosolic tRNA(Ala) into mitochondria in transgenic plants.单个碱基变化阻止转基因植物中胞质丙氨酸转运RNA导入线粒体。
Plant J. 1996 Nov;10(5):913-8. doi: 10.1046/j.1365-313x.1996.10050913.x.
9
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10
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Mol Gen Genet. 1996 Sep 25;252(4):404-11. doi: 10.1007/BF02173005.