Maréchal-Drouard L, Weil J H, Guillemaut P
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
Nucleic Acids Res. 1988 Jun 10;16(11):4777-88. doi: 10.1093/nar/16.11.4777.
Four P. Vulgaris mitochondrial tRNA(Leu) species have been shown to be nuclear encoded. These mt tRNAs(Leu) can be used for in vitro protein synthesis. We found that the sequences of P. vulgaris mitochondrial and cytoplasmic tRNAs(Leu)(NAG) are identical except for a post-transcriptional modification occurring at position 18 (Gm in mt tRNA(Leu) instead of G in cyt tRNA(Leu], as in the case of mt and cyt tRNAs(Leu)(NAA) already sequenced. This post-transcriptional modification has also been found in two other bean mt tRNA(Leu) species, but not in P. vulgaris cytoplasmic tRNA(Leu) species that we have purified so far. Furthermore, comparison of the 2-D polyacrylamide gel electrophoretic patterns of tRNAs eluted from bean mt tRNA-mtDNA and mt tRNA-nDNA hybrids revealed at least 8 mt tRNAs coded for by the nuclear genome.
已证明普通菜豆的四种线粒体tRNA（Leu）由核基因编码。这些线粒体tRNA（Leu）可用于体外蛋白质合成。我们发现，普通菜豆线粒体和细胞质tRNA（Leu）（NAG）的序列除了在第18位发生转录后修饰外是相同的（线粒体tRNA（Leu）中的Gm而不是细胞质tRNA（Leu）中的G，就像已测序的线粒体和细胞质tRNA（Leu）（NAA）的情况一样）。这种转录后修饰也在另外两种菜豆线粒体tRNA（Leu）中发现，但在我们目前纯化的普通菜豆细胞质tRNA（Leu）中未发现。此外，对从菜豆线粒体tRNA-线粒体DNA和线粒体tRNA-核DNA杂交体中洗脱的tRNA的二维聚丙烯酰胺凝胶电泳图谱进行比较，发现至少有8种线粒体tRNA由核基因组编码。
