Kagan J, Liu J, Stein J D, Wagner S S, Babkowski R, Grossman B H, Katz R L
Division of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston 77030, USA.
Oncogene. 1998 Feb 19;16(7):909-13. doi: 10.1038/sj.onc.1201606.
A limited number of previous studies have indicated a low frequency of chromosome 10 allele losses and deletions in bladder cancers. We investigated the involvement of chromosome 10 in advanced bladder cancers. Loss of heterozygosity (LOH) was analysed in 19 microsatellite loci in 20 grade III invasive transitional cell carcinomas. Nine (45%) of the 20 tumors had at least one allele loss on the long arm of chromosome 10. The short arm of chromosome 10 was not affected. The most frequent LOH occurred at D10S215, where four (29%) of 14 of the informative cases had an allele loss. The minimal region with allele losses was located between the centromeric marker D10S1644 and the telomeric marker D10S541, which are separated by 2.52 cM. The results strongly suggest the existence within that region of a tumor suppressor gene or genes for advanced bladder cancer.
此前数量有限的研究表明，膀胱癌中10号染色体等位基因缺失和缺失情况的发生率较低。我们调查了10号染色体在晚期膀胱癌中的作用。对20例III级浸润性移行细胞癌的19个微卫星位点进行了杂合性缺失（LOH）分析。20个肿瘤中有9个（45%）在10号染色体长臂上至少有一个等位基因缺失。10号染色体短臂未受影响。最常见的LOH发生在D10S215，在14例信息性病例中有4例（29%）出现等位基因缺失。等位基因缺失的最小区域位于着丝粒标记D10S1644和端粒标记D10S541之间，二者相距2.52 cM。结果强烈提示，在该区域内存在一个或多个晚期膀胱癌抑癌基因。
