Meineke I, Schreeb K, Kress I, Gundert-Remy U
Department of Clinical Pharmacology, University of Goettingen, Germany.
Ther Drug Monit. 1998 Feb;20(1):14-9. doi: 10.1097/00007691-199802000-00003.
A robust and rapid high-performance liquid chromatography (HPLC) method is described for therapeutic drug monitoring of fluoxetine and norfluoxetine in the presence of six frequently-used tricyclic antidepressants and their respective metabolites. Liquid-liquid extraction into n-hexane/acetonitrile is used with reextraction into hydrochloric acid for clean-up. The chromatographic separation is carried out on a CN column. The minimum detectable amount is 3 ng injected on column. In addition to qualitative and quantitative validation data for the assay method, results from patient samples are presented. It is concluded that for patients treated with fluoxetine, therapeutic drug monitoring is valuable for optimizing the therapy.
本文描述了一种稳健且快速的高效液相色谱（HPLC）方法，用于在六种常用三环类抗抑郁药及其各自代谢物存在的情况下对氟西汀和去甲氟西汀进行治疗药物监测。采用正己烷/乙腈液 - 液萃取，并重新萃取到盐酸中进行净化。色谱分离在CN柱上进行。柱上进样的最低检测量为3 ng。除了该分析方法的定性和定量验证数据外，还给出了患者样本的结果。结论是，对于接受氟西汀治疗的患者，治疗药物监测对于优化治疗具有重要价值。
