Nam J S, Lee D H, Lee K H, Park H M, Bae K S
Genetic Resources Center, KRIBB, Taejon, South Korea.
FEMS Microbiol Lett. 1998 Feb 1;159(1):77-84. doi: 10.1111/j.1574-6968.1998.tb12844.x.
Degenerated PCR primers were used to amplify chitin synthase genes from genomic DNA of Metarhizium anisopliae var. anisopliae. Through cloning and sequencing of approximately 600-bp fragments amplified by PCR, we found three genes encoding different types of chitin synthases, designated MaCHS1, MaCHS2, and MaCHS3. Southern blot analysis performed on genomic DNA showed that each of the chitin synthases MaCHS1, MaCHS2, and MaCHS3 is encoded by a single copy gene. Alignment of their deduced amino acid sequences with those of other euascomycetes separated the sequences into three distinct classes. MaCHS1 was identified as a gene for class I chitin synthase, MaCHS2 for class II, and MaCHS3 for class III. The UPGMA dendrogram and phylogenetic tree of the deduced amino acid sequences revealed the taxonomic and evolutionary position of Metarhizium anisopliae var. anisopliae.
使用简并PCR引物从绿僵菌变种金龟子绿僵菌的基因组DNA中扩增几丁质合成酶基因。通过对PCR扩增的约600bp片段进行克隆和测序，我们发现了三个编码不同类型几丁质合成酶的基因，分别命名为MaCHS1、MaCHS2和MaCHS3。对基因组DNA进行的Southern印迹分析表明，几丁质合成酶MaCHS1、MaCHS2和MaCHS3中的每一个都由单拷贝基因编码。将它们推导的氨基酸序列与其他真子囊菌的序列进行比对，可将这些序列分为三个不同的类别。MaCHS1被鉴定为I类几丁质合成酶的基因，MaCHS2为II类，MaCHS3为III类。推导的氨基酸序列的UPGMA树状图和系统发育树揭示了金龟子绿僵菌变种金龟子绿僵菌的分类学和进化位置。
