Dietrich G, Bubert A, Gentschev I, Sokolovic Z, Simm A, Catic A, Kaufmann S H, Hess J, Szalay A A, Goebel W
Lehrstuhl für Mikrobiologie, University of Würzburg, Germany.
Nat Biotechnol. 1998 Feb;16(2):181-5. doi: 10.1038/nbt0298-181.
Eukaryotic expression vectors can be delivered to macrophages using attenuated self-destructing Listeria monocytogenes. L. monocytogenes cells are preferentially lysed in the host cell macrophage cytosol by the production of a PactA-dependent Listeria-specific phage lysin. Efficient expression of the cloned reporter genes by the macrophages and subsequent antigen presentation were achieved after the delivery of eukaryotic expression vectors by the attenuated suicide L. monocytogenes strain. After delivery by L. monocytogenes plasmid DNAs were found to integrate into the macrophage cell's genome at a frequency of about 10(-7).
真核表达载体可通过减毒的自毁型单核细胞增生李斯特菌递送至巨噬细胞。单核细胞增生李斯特菌细胞在宿主细胞巨噬细胞胞质溶胶中通过产生一种依赖PactA的李斯特菌特异性噬菌体溶素而优先被裂解。在减毒自杀型单核细胞增生李斯特菌菌株递送真核表达载体后，巨噬细胞实现了克隆报告基因的高效表达以及随后的抗原呈递。经单核细胞增生李斯特菌递送后，发现质粒DNA以约10(-7)的频率整合到巨噬细胞基因组中。
