Wang S, Wu H, Jiang J, Delohery T M, Isdell F, Goldman S A
Department of Neurology and Neuroscience, Cornell University Medical Center, New York, NY 10021, USA.
Nat Biotechnol. 1998 Feb;16(2):196-201. doi: 10.1038/nbt0298-196.
Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an alternative strategy for the isolation and enrichment of precursor cells, by fluorescence-activated cell sorting of forebrain cells transfected with the gene for green fluorescent protein, driven by the neuronal T alpha 1 tubulin promoter. Using this approach, neural precursors and young neurons can be identified and selectively harvested from a variety of samples, including both avian and mammalian forebrains at different developmental stages.
神经元前体细胞广泛存在于前脑脑室下区，可能为脑修复提供细胞基础。随着时间推移和体外传代，源自单个祖细胞的克隆系可能会逐渐失去其亲代前体细胞的代表性。我们开发了一种分离和富集前体细胞的替代策略，即通过荧光激活细胞分选技术，对由神经元α1微管蛋白启动子驱动的绿色荧光蛋白基因转染的前脑细胞进行分选。利用这种方法，可以从包括不同发育阶段的禽类和哺乳动物前脑在内的各种样本中识别并选择性收获神经前体细胞和年轻神经元。
