Isobe T, Shimizu S, Yoshihara S, Suzuki K
National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Parasitol. 1998 Feb;84(1):62-6.
The humoral antibody responses of chickens infected or immunized with Leucocytozoon caulleryi were analyzed by immunoblot comparing with protection against challenge infection, agar-gel precipitation (AGP) test, enzyme-linked immunosorbent assay (ELISA), and parasitemia. IgG antibodies in the sera from chickens infected with L. caulleryi were found to react with 20-35 bands of approximate molecular weights 25-300 kDa, such as 33, 44, 58, 79, 94, and 141 kDa from 3 to 50 wk after infection. In chickens immunized with schizont antigens from L. caulleryi, several bands were reacted with sera 2 wk after the second immunization, e.g., 36, 58, 71, 81, 97, 112, and 123 kDa. Chickens that recovered from the primary infection showed complete protection against reinfection, whereas immunized chickens showed partial protection against challenge infection. These results suggest that the difference in antibody response to schizont antigen might cause the difference in protection between immunized and infected chickens.
通过免疫印迹法，并与攻毒感染的保护作用、琼脂凝胶沉淀（AGP）试验、酶联免疫吸附测定（ELISA）及虫血症进行比较，分析了感染或免疫卡氏住白细胞虫的鸡的体液抗体反应。发现感染卡氏住白细胞虫的鸡血清中的IgG抗体与大约25至300 kDa分子量的20 - 35条带发生反应，例如感染后3至50周出现的分子量为33、44、58、79、94和141 kDa的条带。在用卡氏住白细胞虫裂殖体抗原免疫的鸡中，第二次免疫后2周，几条带与血清发生反应，例如分子量为36、58、71、81、97、112和123 kDa的条带。从初次感染中恢复的鸡对再次感染表现出完全保护，而免疫鸡对攻毒感染表现出部分保护。这些结果表明，对裂殖体抗原的抗体反应差异可能导致免疫鸡和感染鸡在保护作用上的差异。
