Sullivan J T, Lares R R, Galvan A G
Biology Department, University of the Incarnate Word, San Antonio, Texas 78209, USA.
J Parasitol. 1998 Feb;84(1):82-7.
The posterior tip of juvenile snails, consisting of immature ovotestis, vesicular connective tissue, and digestive gland acini, all surrounded by body wall, was implanted into the hemocoel of allogeneic adult Biomphalaria glabrata. Recipient snails were either uninfected or had been infected 15 days previously with Schistosoma mansoni. Graft recipients were dissected at 30 or 60 days postimplantation (DPI), and the implants, along with preimplantation controls, were examined histologically. The gonads in preimplantation controls consisted of a partially coiled collecting canal partitioned dorsally by connective tissue septa and contained mainly spermatogonia-like cells and small oocytes. In uninfected recipients, implanted gonads underwent rapid development, i.e., had formed mature acini and were carrying out vigorous gametogenesis, both spermatogenesis and oogenesis, by 30 DPI. At 60 DPI, numbers of spermatozoa had increased substantially in most implants. In infected recipients, relatively little development or gametogenesis occurred at either 30 or 60 DPI, and oogenesis was more strongly inhibited than spermatogenesis. Except for 1 implanted gonad (out of 25) that had undergone necrosis at 60 DPI and a second otherwise healthy 60-day implant partially encapsulated at its cut surface (both in uninfected recipients), no significant hemocytic response occurred against the allografts.
将幼体蜗牛的后端，即由未成熟的卵精巢、泡状结缔组织和消化腺腺泡组成，且全部被体壁包围的部分，植入同种异体成年光滑双脐螺的血腔中。受体蜗牛要么未感染，要么在15天前已感染曼氏血吸虫。在植入后30天或60天（DPI）解剖移植受体，并对植入物以及植入前的对照进行组织学检查。植入前对照中的性腺由一个部分盘绕的收集管组成，该收集管在背侧被结缔组织隔膜分隔，主要包含精原细胞样细胞和小卵母细胞。在未感染的受体中，植入的性腺迅速发育，即在30 DPI时已形成成熟的腺泡，并正在进行活跃的配子发生，包括精子发生和卵子发生。在60 DPI时，大多数植入物中的精子数量大幅增加。在感染的受体中，在30 DPI或60 DPI时相对很少发生发育或配子发生，并且卵子发生比精子发生受到更强的抑制。除了1个植入的性腺（25个中的）在60 DPI时发生坏死，以及另一个在60天时原本健康的植入物在其切割表面部分被包裹（均在未感染的受体中）外，未对同种异体移植产生明显的血细胞反应。
