黏附和非黏附性大肠杆菌的D-乳酸生成及[14C]琥珀酸摄取
D-lactate production and [14C]succinic acid uptake by adherent and nonadherent Escherichia coli.
作者信息
McCabe K, Mann M D, Bowie M D
机构信息
Institute of Child Health, University of Cape Town, Red Cross War Memorial Children's Hospital, Rondebosch, South Africa.
出版信息
Infect Immun. 1998 Mar;66(3):907-11. doi: 10.1128/IAI.66.3.907-911.1998.
Abstract
Escherichia coli isolates of different adherence phenotypes produced different amounts of D-lactate. Alterations of culture conditions did not influence the amount of D-lactate produced. The observed pH decreases in tissue culture medium corresponded with increases in D-lactate concentration. Very little [14C]succinic acid was incorporated into cells during the in vitro incubation of adherent and nonadherent E. coli with HeLa cells, but the amounts of tracer removed from the culture medium by adherent and nonadherent strains differed. The results are further evidence of a difference in the metabolic behavior of adherent and nonadherent E. coli.
摘要
不同黏附表型的大肠杆菌分离株产生的D-乳酸量不同。培养条件的改变不影响D-乳酸的产生量。在组织培养基中观察到的pH下降与D-乳酸浓度的增加相对应。在用HeLa细胞对黏附性和非黏附性大肠杆菌进行体外培养期间，很少有[14C]琥珀酸掺入细胞，但黏附性和非黏附性菌株从培养基中去除的示踪剂数量不同。这些结果进一步证明了黏附性和非黏附性大肠杆菌在代谢行为上的差异。
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