Dolzhanskiy A, Hirst J, Basch R S, Karpatkin S
Department of Pathology, New York University Medical Center, New York 10016, USA.
Br J Haematol. 1998 Feb;100(2):415-26. doi: 10.1046/j.1365-2141.1998.00579.x.
The effect of IL-3 on the early steps in the growth and development of megakaryocytes (MK) in culture has been studied. Although thrombopoietin (TPO) by itself could support the development of mature CD41+ MK from pre-MK, the number of cells produced was greatly augmented by the addition of IL-3 and SCF. IL-3 was also able to support the growth of MK colonies in semi-solid media (CFU-MK). The CD41+ cells that developed in suspension cultures containing IL-3 differed phenotypically from those that developed without this agent. Cells grown in the presence of IL-3 lost CD34 expression more rapidly, expressed lower levels of the platelet glycoproteins gpIIb-IIIa and Ib and achieved lower degrees of polyploidy than in the absence of IL-3. The inhibitory effects of IL-3 were not a consequence of the dilution of the mature cells by increased numbers of immature cells since it was observed under conditions in which IL-3 did not stimulate MK growth. The results obtained in these cultures suggest that IL-3 plays an important role in early MK development, but inhibits further maturation after endoreduplication begins. Thus, prolonged contact with IL-3 results in the appearance of cells that do not mature normally.
研究了白细胞介素-3（IL-3）对体外培养的巨核细胞（MK）生长和发育早期阶段的影响。尽管血小板生成素（TPO）本身能够支持前巨核细胞发育为成熟的CD41+巨核细胞，但添加IL-3和干细胞因子（SCF）可显著增加所产生的细胞数量。IL-3还能够支持半固体培养基中巨核细胞集落（CFU-MK）的生长。在含有IL-3的悬浮培养物中发育的CD41+细胞在表型上与不添加该因子时发育的细胞不同。与无IL-3时相比，在有IL-3存在的情况下生长的细胞更快地失去CD34表达，血小板糖蛋白gpIIb-IIIa和Ib表达水平更低，多倍体程度也更低。IL-3的抑制作用并非由于未成熟细胞数量增加导致成熟细胞被稀释，因为在IL-3不刺激巨核细胞生长的条件下也观察到了这种现象。这些培养实验结果表明，IL-3在巨核细胞早期发育中起重要作用，但在内复制开始后会抑制其进一步成熟。因此，长时间接触IL-3会导致出现不能正常成熟的细胞。
