Murphy G J, Leavitt A D
Departments of Laboratory Medicine and Internal Medicine, University of California, San Francisco, CA 94143, USA.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3065-70. doi: 10.1073/pnas.96.6.3065.
The limited current understanding of megakaryocyte-lineage development and megakaryocyte biology is in large part because of a paucity of useful systems in which to conduct experiments. To overcome this problem, we have developed a transgenic mouse that uses the GP-Ibalpha regulatory sequences to achieve megakaryocyte-lineage restricted expression of an avian retroviral receptor. Through the transgenic avian receptor, avian retroviruses can efficiently and selectively infect megakaryocyte-lineage cells in vitro and in vivo. Serial infections can be performed to introduce and express multiple genes in the same cell. We have used this system to generate and characterize a pure population of primary CD41-positive megakaryocyte progenitors.
目前对巨核细胞系发育和巨核细胞生物学的了解有限，很大程度上是因为缺乏用于进行实验的有用系统。为了克服这个问题，我们开发了一种转基因小鼠，它利用糖蛋白Ibα（GP-Ibalpha）调控序列来实现禽逆转录病毒受体在巨核细胞系中的限制性表达。通过转基因禽受体，禽逆转录病毒可以在体外和体内有效且选择性地感染巨核细胞系细胞。可以进行连续感染以在同一细胞中引入和表达多个基因。我们已经使用这个系统来生成并鉴定了一群纯的原代CD41阳性巨核细胞祖细胞。
