Ferrari G, Cusella-De Angelis G, Coletta M, Paolucci E, Stornaiuolo A, Cossu G, Mavilio F
H. San Raffaele-Telethon Institute for Gene Therapy (TIGET), 20132 Milan, Italy.
Science. 1998 Mar 6;279(5356):1528-30. doi: 10.1126/science.279.5356.1528.
Growth and repair of skeletal muscle are normally mediated by the satellite cells that surround muscle fibers. In regenerating muscle, however, the number of myogenic precursors exceeds that of resident satellite cells, implying migration or recruitment of undifferentiated progenitors from other sources. Transplantation of genetically marked bone marrow into immunodeficient mice revealed that marrow-derived cells migrate into areas of induced muscle degeneration, undergo myogenic differentiation, and participate in the regeneration of the damaged fibers. Genetically modified, marrow-derived myogenic progenitors could potentially be used to target therapeutic genes to muscle tissue, providing an alternative strategy for treatment of muscular dystrophies.
骨骼肌的生长和修复通常由围绕肌纤维的卫星细胞介导。然而，在再生肌肉中，生肌前体细胞的数量超过了驻留卫星细胞的数量，这意味着未分化祖细胞从其他来源迁移或被招募。将基因标记的骨髓移植到免疫缺陷小鼠中发现，骨髓来源的细胞迁移到诱导性肌肉退化区域，进行生肌分化，并参与受损纤维的再生。基因改造的骨髓来源的生肌祖细胞有可能用于将治疗性基因靶向肌肉组织，为治疗肌肉萎缩症提供一种替代策略。
