Totzauer I, Kölle S, Sinowatz F, Plendl J, Amselgruber W, Töpfer-Petersen E
Lehrstuhl für Tieranatomie II, LMU München, Germany.
Ann Anat. 1998 Feb;180(1):37-43. doi: 10.1016/s0940-9602(98)80128-3.
The purpose of this study was to investigate the expression of two zona pellucida gene families ZPB (ZP3 alpha) and ZPC (ZP3 beta). Sections of ovaries from bovine fetuses, calves and cows were labelled with polyclonal antibodies. Immunopositive labelling was found in both the follicle cells and the oocyte. Labelling was dependent on the stage of development. The specific sequence of immunopositive reactions suggests that in the bovine fetus both the ovary and the follicle cells contribute to the production of the zona pellucida during pre- and postnatal development.
本研究的目的是调查两个透明带基因家族ZPB（ZP3α）和ZPC（ZP3β）的表达情况。用多克隆抗体对牛胎儿、犊牛和母牛的卵巢切片进行标记。在卵泡细胞和卵母细胞中均发现免疫阳性标记。标记取决于发育阶段。免疫阳性反应的特定序列表明，在牛胎儿中，卵巢和卵泡细胞在产前和产后发育过程中都参与透明带的产生。
