Törmälä Reeta-Maria, Jääskeläinen Minna, Lakkakorpi Jouni, Liakka Annikki, Tapanainen Juha S, Vaskivuo Tommi E
Department of Obstetrics and Gynaecology, University of Oulu, and Clinical Research Center, Oulu University Hospital, Oulu, Finland.
Mol Cell Endocrinol. 2008 Jul 16;289(1-2):10-5. doi: 10.1016/j.mce.2008.01.029. Epub 2008 Mar 10.
The zona pellucida is a glycoprotein matrix surrounding oocytes and early-stage embryos in mammals. To elucidate the roles of the zona pellucida glycoproteins ZP1 and ZP3 and their key regulatory factor FIGLA in human ovarian development and folliculogenesis, their expression and localization was studied in human fetal and adult ovaries. FIGLA mRNA and ZP3 mRNA/protein were localized mainly in the oocytes, and during fetal development their maximal expression was observed around the 20th week, the time of follicle formation. The expression of ZP1 mRNA was low both in fetal and adult ovaries. Present findings demonstrate that ZP3 and FIGLA transcripts are expressed in the oocytes from early ovarian development. The function of ZP proteins during early fetal life is not clear, but the simultaneous expression of FIGLA and ZP3 suggests, that they may have a role in the development of primordial follicle before zona pellucida formation.
透明带是围绕哺乳动物卵母细胞和早期胚胎的糖蛋白基质。为了阐明透明带糖蛋白ZP1和ZP3及其关键调节因子FIGLA在人类卵巢发育和卵泡发生中的作用，研究了它们在人类胎儿和成人卵巢中的表达和定位。FIGLA mRNA和ZP3 mRNA/蛋白主要定位于卵母细胞中，在胎儿发育期间，它们的最大表达在第20周左右观察到，这是卵泡形成的时间。ZP1 mRNA在胎儿和成人卵巢中的表达均较低。目前的研究结果表明，ZP3和FIGLA转录本在卵巢早期发育的卵母细胞中表达。ZP蛋白在胎儿早期的功能尚不清楚，但FIGLA和ZP3的同时表达表明，它们可能在透明带形成之前的原始卵泡发育中起作用。
