Inoue H K, Shirao T
J Electron Microsc (Tokyo). 1997;46(6):497-502. doi: 10.1093/oxfordjournals.jmicro.a023549.
Ultrastructural changes associated with neurite formation were examined in suitably cultured neuroblastoma cells (Neuro-2a) and drebrin (developmentally regulated brain protein) gene-transfected fibroblasts (L cells) in culture. Both neuroblastoma cells and fibroblasts initially were flattened and epitheloid, with many microspikes or microvilli diffusely distributed over their surfaces. Intracellular organelles were abundant and diffusely arranged. In the transformed state, both cell types were round to oval with long processes where microspikes were concentrated. A constant arrangement of surface and intracellular structures was apparent, though drebrin gene-transfected fibroblasts retained some of their original characteristics. Neurite formation programmed by genes may be initiated by environmental factors in neuroblastoma cells. Neurite-like processes in fibroblasts may be formed due to changes in microfilaments resulting from transfection of the drebrin (actin-binding protein) gene.
在体外培养的合适的神经母细胞瘤细胞（Neuro-2a）和转染了drebrin（发育调控脑蛋白）基因的成纤维细胞（L细胞）中，研究了与神经突形成相关的超微结构变化。神经母细胞瘤细胞和成纤维细胞最初都是扁平的上皮样细胞，表面广泛分布着许多微刺或微绒毛。细胞内细胞器丰富且分布弥散。在转化状态下，两种细胞类型均呈圆形至椭圆形，带有长突起，微刺集中于此。尽管转染了drebrin基因的成纤维细胞保留了一些原始特征，但表面和细胞内结构的恒定排列是明显的。基因编程的神经突形成可能由神经母细胞瘤细胞中的环境因素引发。成纤维细胞中的神经突样突起可能是由于转染drebrin（肌动蛋白结合蛋白）基因导致微丝变化而形成的。
