Suzuki K, Itai R, Suzuki K, Nakanishi H, Nishizawa N K, Yoshimura E, Mori S
Department of Applied Biological Chemistry, University of Tokyo, Japan.
Plant Physiol. 1998 Feb;116(2):725-32. doi: 10.1104/pp.116.2.725.
To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel electrophoresis. Peptide sequence analysis of induced proteins revealed that formate dehydrogenase (FDH), adenine phosphoribosyltransferase, and the lds3 gene product (for Fe deficiency-specific) increased in Fe-deficient roots. FDH enzyme activity was detected in Fe-deficient roots but not in Fe-sufficient roots. A cDNA encoding FDH (Fdh) was cloned and sequenced. Fdh expression was induced by Fe deficiency. Fdh was also expressed under anaerobic stress and its expression was more rapid than that induced by Fe deficiency. Thus, the expression of Fdh observed in Fe-deficient barley roots appeared to be a secondary effect caused by oxygen deficiency in Fe-deficient plants.
为了鉴定缺铁诱导产生的蛋白质，我们通过二维聚丙烯酰胺凝胶电泳比较了铁充足和缺铁大麦（Hordeum vulgare L.）根系的蛋白质。对诱导蛋白的肽序列分析表明，甲酸脱氢酶（FDH）、腺嘌呤磷酸核糖转移酶和lds3基因产物（缺铁特异性）在缺铁根系中增加。在缺铁根系中检测到了FDH酶活性，而在铁充足的根系中未检测到。克隆并测序了编码FDH（Fdh）的cDNA。Fdh的表达受缺铁诱导。Fdh在厌氧胁迫下也有表达，且其表达比缺铁诱导的表达更快。因此，在缺铁大麦根系中观察到的Fdh表达似乎是缺铁植物中缺氧引起的次生效应。
