Rubtsova E R, Kovalev I E
Zh Mikrobiol Epidemiol Immunobiol. 1976 Jun(6):29-32.
In repeated injections of 6-mercaptopurine (6-MP) covalently bound with protein (6-MP-P) antibodies binding 6-MP appeared in rabbits. In such animals free 6-MP produced a lesser immunodepressive and toxic action. Injection to mice immunized with sheep erythrocytes (SE) of rabbit serum containing antibodies against 6-MP not only failed to lead to reduction of the immunodepressive action of this cytostatic, but, on the contrary, intensified the inhibition of antibody formation to the SE. Injection to mice of 6-MP bound with rabbit antiserum in vitro also enhanced its immunodepressive activity. Evidently own antibodies neutralized 6-MP, whereas foreign ones, serving as an antigen, apparently caused its accumulation in the macrophagal lymphocytic complex.
在多次注射与蛋白质共价结合的6-巯基嘌呤（6-MP-P）后，兔体内出现了结合6-MP的抗体。在这类动物中，游离的6-MP产生的免疫抑制和毒性作用较小。给用绵羊红细胞（SE）免疫的小鼠注射含抗6-MP抗体的兔血清，不仅未能导致这种细胞抑制剂的免疫抑制作用减弱，反而增强了对SE抗体形成的抑制。体外将6-MP与兔抗血清结合后注射到小鼠体内也增强了其免疫抑制活性。显然，自身抗体中和了6-MP，而作为抗原的外来抗体显然导致其在巨噬细胞淋巴细胞复合物中积累。
