Stetsenko O G, Baĭzhomartov M S, Irzhanov S D
Zh Mikrobiol Epidemiol Immunobiol. 1976 Jun(6):44-6.
A possibility was shown of cultivation of M. pneumoniae in the organ cultures of the trachea and the lungs of a pig embryo. 5-7-day guinea pigs, and 3-5-day chicks. The lung and tracheal explants of chicks possessed the greatest sensitivity to M. pneumoniae. M. pneumoniae strains were isolated from the nasopharyngeal smears and washings from the bronchi obtained in bronchoscopy of patients suffering from chronic pneumonia, with the use of organ culture of chick trachea. Explains of chick trachea could serve as a medium for the isolation of M. pneumoniae from the patients.
已证实在猪胚胎、5 - 7日龄豚鼠以及3 - 5日龄雏鸡的气管和肺的器官培养物中培养肺炎支原体是可能的。雏鸡的肺和气管外植体对肺炎支原体的敏感性最高。利用雏鸡气管的器官培养,从患有慢性肺炎患者的支气管镜检查获得的鼻咽涂片和支气管冲洗物中分离出了肺炎支原体菌株。雏鸡气管外植体可作为从患者中分离肺炎支原体的培养基。