通透酶同源物Ssy1p控制酿酒酵母中氨基酸和肽转运蛋白基因的表达。

The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae.

作者信息

Didion T, Regenberg B, Jørgensen M U, Kielland-Brandt M C, Andersen H A

机构信息

Department of Yeast Genetics, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, Denmark.

出版信息

Mol Microbiol. 1998 Feb;27(3):643-50. doi: 10.1046/j.1365-2958.1998.00714.x.

DOI:10.1046/j.1365-2958.1998.00714.x

PMID:9489675

Abstract

Amino acid transporters of the yeast plasma membrane (permeases) belong to a family of integral membrane proteins with pronounced structural similarity. We present evidence that a member of this family, encoded by the open reading frame (ORF) YDR160w (SSY1), is required for the expression of a set of transporter genes. Thus, deletion of the SSY1 gene causes loss of leucine-inducible transcription of the amino acid permease genes BAP2, TAT1 and BAP3 (ORF YDR046c) and the peptide transporter, PTR2. D-leucine can generate the signal without entering the cell. We propose that Ssy1p is situated in the plasma membrane and is involved in sensing leucine in the medium.

摘要

酵母质膜的氨基酸转运蛋白（通透酶）属于一类具有明显结构相似性的整合膜蛋白家族。我们提供的证据表明，这个家族的一个成员，由开放阅读框（ORF）YDR160w（SSY1）编码，是一组转运蛋白基因表达所必需的。因此，缺失SSY1基因会导致氨基酸通透酶基因BAP2、TAT1和BAP3（ORF YDR046c）以及肽转运蛋白PTR2的亮氨酸诱导转录丧失。D-亮氨酸无需进入细胞就能产生信号。我们推测Ssy1p位于质膜中，并参与感知培养基中的亮氨酸。