Katzenellenbogen E, Kocharova N A, Zatonsky G V, Mieszala M, Gamian A, Bogulska M, Shashkov A S, Romanowska E, Knirel Y A
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Eur J Biochem. 1998 Feb 1;251(3):980-5. doi: 10.1046/j.1432-1327.1998.2510980.x.
On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O-specific polysaccharide (O-PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic-acid-like polymer having a repeating unit of the following structure: [structure in text] where Qui4NAc is 4-acetamido-4,6-dideoxyglucose and O-acetylation at both positions is non-stoichiometric. The glycosidic linkage of the lateral beta-D-GlcpNAc residue is acid-labile and cleaved from the O-PS during mild acid hydrolysis or dephosphorylation with 48% hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O-PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral alpha-D-Glcp residue and the position of one of the O-acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O-PSs of the two H. alvei strains.
基于化学分析和核磁共振光谱研究，发现从蜂房哈夫尼亚菌PCM 1199脂多糖（LPS）中分离出的O-特异性多糖（O-PS）是一种类甘油磷壁酸聚合物，其具有以下结构的重复单元：[文中结构]，其中Qui4NAc是4-乙酰氨基-4,6-二脱氧葡萄糖，两个位置的O-乙酰化是非化学计量的。外侧β-D-GlcpNAc残基的糖苷键对酸不稳定，在温和酸水解或用48%氢氟酸脱磷酸化过程中从O-PS上断裂。比较分析表明，蜂房哈夫尼亚菌PCM 1199 O-PS的结构与蜂房哈夫尼亚菌PCM 1205的结构相似，后者仅在存在一个额外的外侧α-D-Glcp残基和一个O-乙酰基的位置上有所不同。因此，血清学测试显示这两种蜂房哈夫尼亚菌菌株的LPS和O-PS之间存在高度的血清学相似性。
