Karásková J, Holena O, Michalová K
Ustav hematologie a krevni transfuze, Praha.
Cas Lek Cesk. 1997 Oct 22;136(20):633-7.
The cytogenetic examination of the bone marrow (BM) depends on the presence of sufficient amount of mitoses on slides prepared for analysis. The quantity and the quality of mitoses found in BM cultivations of patients with MDS is rather low and a new possibilities to increase the proliferative activity of the cells are under research. One promising method is the stimulation of the BM cells with addition of hemopoietic growth factors. The aim of this study was to evaluate in vitro influence of GM-CSF on the proliferative activity and the representation of chromosomally different clones in the patients with MDS.
The bone marrow cells of 25 patients (12 males, 13 females) of mean age 52.5 years (range 20-85) were examined. The mitotic index (MI), quality of mitoses and chromosomal complement were evaluated in 24 and 72 h cultivations with 100 and 1000 ng/ml GM-CSF concentrations. No stimulation was used in control cultivations. After 24 h of cultivation 100 ng/ml of GM-CSF increased the mitotic index (112%) but lowered the quality of mitoses (-7.6%). On contrary after 72 h of presence of 100 ng/ml GM-CSF both parameters showed the decrease (MI-30%, quality of mitoses-36%). Similar effect had the addition of 1000 ng/ml for 24 h of cultivation (MI-5%, quality of mitoses-11%). Twelve patients had normal karyotype on all studied cells in every type of cultivation. The ratio of chromosomally abnormal clones was the same in six other cases and only non-significant changes appeared in seven patients after GM-CSF stimulation.
The influence of GM-CSF on the division of the bone marrow cells is strictly individual. GM-CSF in concentration of 100 ng/ml in 24 h cultivation increases the activity of proliferation, but at the same time moderately decreases the quality of mitoses. The presence of GM-CSF has not changed the cytogenetic results.
骨髓的细胞遗传学检查取决于用于分析的载玻片上是否存在足够数量的有丝分裂细胞。骨髓增生异常综合征（MDS）患者骨髓培养物中发现的有丝分裂细胞的数量和质量相当低，目前正在研究提高细胞增殖活性的新方法。一种有前景的方法是添加造血生长因子来刺激骨髓细胞。本研究的目的是评估粒细胞巨噬细胞集落刺激因子（GM-CSF）对MDS患者细胞增殖活性及染色体不同克隆比例的体外影响。
对25例患者（12例男性，13例女性）的骨髓细胞进行检查，平均年龄52.5岁（范围20 - 85岁）。在含有100和1000 ng/ml GM-CSF浓度的培养物中培养24小时和72小时后，评估有丝分裂指数（MI）、有丝分裂质量和染色体组成。对照培养物未使用刺激剂。培养24小时后，100 ng/ml的GM-CSF使有丝分裂指数增加（112%），但有丝分裂质量下降（-7.6%）。相反，在含有100 ng/ml GM-CSF培养72小时后，这两个参数均下降（MI - 30%，有丝分裂质量 - 36%）。培养24小时添加1000 ng/ml GM-CSF也有类似效果（MI - 5%，有丝分裂质量 - 11%）。12例患者在每种培养类型的所有研究细胞中核型均正常。其他6例患者染色体异常克隆的比例相同，GM-CSF刺激后7例患者仅出现非显著变化。
GM-CSF对骨髓细胞分裂的影响具有严格的个体差异。培养24小时时，100 ng/ml浓度的GM-CSF可增加增殖活性，但同时适度降低有丝分裂质量。GM-CSF的存在并未改变细胞遗传学结果。
