Jahnsen F L, Farstad I N, Aanesen J P, Brandtzaeg P
Laboratory for Immunohistochemistry and Immunopathology, Institute of Pathology, Rikshospitalet, Oslo, Norway.
Am J Respir Cell Mol Biol. 1998 Mar;18(3):392-401. doi: 10.1165/ajrcmb.18.3.2995.
Phenotypic and functional studies are required to understand the immunoregulatory role of mucosal T cells. Information about T cells in the human upper respiratory tract is limited and conflicting. Therefore, we phenotyped T cells in nasal mucosa by means of multicolor in situ immunofluorescence. In normal mucosa, most CD3+ intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) (> 90%) expressed T-cell receptor (TCR)alpha/beta, and only approximately 5% expressed TCRgamma/delta. Although most IELs in the surface epithelium were CD8+ (64%), many expressed CD4 (30%) and the CD4 phenotype dominated (55%) only slightly in the lamina propria. This result was strikingly different from that obtained for comparable compartments in histologically normal jejunal mucosa, where IELs consisted of 83% CD8+ and LPLs of 73% CD4(+) T cells. Nasal CD3+ IELs and LPLs were mainly CD45RO+CD45RA- and usually expressed CD7. The integrin alphaEbeta7 was, as expected, more common on IELs than on LPLs (78 versus 20%). In conclusion, nasal T cells show several similarities to those of the normal jejunum but some notable differences exist, especially a relative increase in CD4+ T cells in the epithelium and a decrease in the lamina propria. It should be explored whether this disparity, together with an increased expression of epithelial adhesion molecules, might contribute to local immunological overstimulation and partly explain the relatively high frequency of airway allergy.
需要进行表型和功能研究以了解黏膜T细胞的免疫调节作用。关于人类上呼吸道T细胞的信息有限且相互矛盾。因此，我们通过多色原位免疫荧光对鼻黏膜中的T细胞进行了表型分析。在正常黏膜中，大多数CD3⁺上皮内淋巴细胞（IELs）和固有层淋巴细胞（LPLs）（>90%）表达T细胞受体（TCR）α/β，只有约5%表达TCRγ/δ。虽然表面上皮中的大多数IELs为CD8⁺（64%），但许多表达CD4（30%），且CD4表型仅在固有层中略占主导（55%）。这一结果与组织学正常的空肠黏膜中类似区域的结果显著不同，在空肠黏膜中，IELs由83%的CD8⁺和73%的CD4⁺T细胞组成。鼻CD3⁺IELs和LPLs主要为CD45RO⁺CD45RA⁻，通常表达CD7。正如预期的那样，整合素αEβ7在IELs上比在LPLs上更常见（78%对20%）。总之，鼻T细胞与正常空肠的T细胞有一些相似之处，但也存在一些显著差异，特别是上皮中CD4⁺T细胞相对增加，固有层中减少。应探讨这种差异以及上皮黏附分子表达的增加是否可能导致局部免疫过度刺激，并部分解释气道过敏的相对高发生率。
